AP21490HR-N Rabbit IgA+IgG+IgM (H+L chain) antibody

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1 ml / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP21490HR-N

Product Description for Rabbit IgA+IgG+IgM

Properties: (H+L chain)
Presentation: HRP
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Rabbit IgA+IgG+IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Rabbit IgA, Rabbit IgG, Rabbit IgM
Presentation HRP
Reactivity Rb
Applications C, Dot, E, ICC/IF, ID, IP, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified immunoglobulin fractions isolated from pooled rabbit serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Background The intended use of this antibody is the detection of IgG by enzyme-immunocytochemical and immunohistochemical staining (antigen or antibody) of appropriately treated cell and tissue substrates at the cellular and subcellular level, or by non-isotopic assay methodology.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life
HRP
Molar radio:
Peroxidase/IgG: ~1.7
Purification:
Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt-Precipitation and purifaction of the IgG fraction by DEAE Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore by adding 1.0 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in rabbit serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
Cross-Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
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