AP21490HR-N Rabbit IgA+IgG+IgM (H+L chain) antibody

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1 ml / €450.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Rabbit IgA+IgG+IgM

Properties: (H+L chain)
Presentation: HRP
Product is tested for Paraffin Sections, Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Rabbit IgA+IgG+IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Rabbit IgA, Rabbit IgG, Rabbit IgM
Presentation HRP
Reactivity Rb
Applications C, Dot, E, ICC/IF, ID, IP, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified immunoglobulin fractions isolated from pooled rabbit serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Background The intended use of this antibody is the detection of IgG by enzyme-immunocytochemical and immunohistochemical staining (antigen or antibody) of appropriately treated cell and tissue substrates at the cellular and subcellular level, or by non-isotopic assay methodology.
Concentration appr. 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Horseradish Peroxidase
Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life
Molar radio:
Peroxidase/IgG: ~1.7
Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt-Precipitation and purifaction of the IgG fraction by DEAE Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
Lyophilized purified IgG fraction
Restore by adding 1.0 ml sterile distilled water.
The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in rabbit serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
Cross-Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
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