discontinued

NB120-7171 Rabbit IgG Fab Fragment (Heavy and Light chain) antibody

Search for all "Rabbit IgG Fab Fragment"

0.5 mg / €270.00

NB120-7171

Product Description for Rabbit IgG Fab Fragment

Properties: (Heavy and Light chain)
Presentation: HRP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Rabbit IgG Fab Fragment

Product Category Secondary Antibodies
Quantity 0.5 mg
Synonyms Rabbit Immunoglobulin G
Presentation HRP
Reactivity Rb
Applications E, P, WB
Clonality Polyclonal
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Rabbit IgG whole molecule
Add. information Dot: Use at an assay dependent dilution. Electron Microscopy: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/8000 - 1/34000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/8000 to 1/34000 of the reconstitution concentration is suggested for this product.Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunofluorescence, immunohistochemistry, Western Blot, ELISA, Immunohistochemistry, Immunofluorescence and Western Blot: Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications. Optimal dilutions and concentrations should be determined by the end user., ELISA 1/8000 - 1/34000, immunohistochemistry assay dependent, immunofluorescence assay dependent, Western Blot assay dependent
Concentration 1 mg/ml
General Readings General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). PubMed PMID: 6172519
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
HRP
Purification:
Affinity purified
Buffer System:
0.01% Gentamicin Sulfate
Purity:
Affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Species:
Rabbit
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