R1364F Rabbit IgG (H+L chain) antibody

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2 mg / €200.00
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R1364F

Product Description for Rabbit IgG

Properties: (H+L chain)
Presentation: FITC
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence.

Properties for Rabbit IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Rabbit Immunoglobulin G
Presentation FITC
Reactivity Rb
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rabbit IgG, whole molecule.
Application This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluoresence: 1/1,000-1/5,000.
Fluorescent Western Blot.
Concentration 2.0 mg/ml (after reconstitution) by UV absorbance at 280 nm
Product Citations
Purchased from Acris:
HRP conjugated antibody is cited in:
  1. Kokk K, Kuuslahti M, Keisala T, Purmonen S, Kaipia A, Tammela T, et al. Expression of luteinizing hormone receptors in the mouse penis. J Androl. 2011 Jan-Feb;32(1):49-54. doi: 10.2164/jandrol.109.008623. Epub 2010 Aug 26. PubMed PMID: 20798391.
General Readings
  1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage Store vial at 2-8°C prior to restoration.
This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after restoration and standing at RT.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Protect from light.
Shelf life: One year from despatch.
Format
Label:
Fluorescein isothiocyanate (Molecular Weight 390 daltons)
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
3.7 moles FITC per mole of Goat IgG
Purification:
Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
10 mg/ml BSA (Ig and Protease free)
State:
Lyophilized purified Ig fraction
Lyophilized
Reconstitution:
Restore with 1.0 ml of deionized water or equivalent.
Species Reactivity
Species reactivity (tested):
Rabbit.
Specificity
Specificity:
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
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