R1364HRP Rabbit IgG (H+L chain) antibody

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2 mg / €220.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Product Description for Rabbit IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Paraffin Sections, Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Rabbit IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Rabbit Immunoglobulin G
Presentation HRP
Reactivity Rb
Applications E, P, WB
Clonality Polyclonal
Host Goat
Molecular weight 40 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rabbit IgG, whole molecule
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000-1/250,000.
Western blot: 1/10,000-1/20,000.
Immunohistochemistry: 1/500-1/2,500.
Background Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) is available in a variety of formats. HRP Anti IgG secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other HRP antibody based assays.
Caution Do Not Add Sodium Azide.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Kokk K, Kuuslahti M, Keisala T, Purmonen S, Kaipia A, Tammela T, et al. Expression of luteinizing hormone receptors in the mouse penis. J Androl. 2011 Jan-Feb;32(1):49-54. doi: 10.2164/jandrol.109.008623. Epub 2010 Aug 26. PubMed PMID: 20798391.
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144, 1981. (Conjugation)
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin Sulfate
Stabilizers:
10 mg/ml BSA (Ig and Protease free)
State:
Lyophilized purified Ig fraction
Lyophilized
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
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