R1377AP Rat IgA + IgG + IgM (H+L chain) antibody

Search for all "Rat IgA + IgG + IgM"

1 mg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Rat IgA + IgG + IgM

Properties: (H+L chain)
Presentation: AP

Properties for Rat IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 1 mg
Presentation AP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Rat IgG, IgA and IgM whole molecule
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilution:
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
Storage Store the antibody (undiluted) at 2-8°C.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Immunoaffinity chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative.
Liquid (sterile filtered) purified Ig fraction.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum.
This reagent is suitable for the detection of all Rat immunoglobulin isotypes, subclasses and chain combinations.
  • LinkedIn