R1377B Rat IgA + IgG + IgM (H+L chain) antibody

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2 mg / €300.00
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R1377B

Product Description for Rat IgA + IgG + IgM

Properties: (H+L chain)
Presentation: Biotin

Properties for Rat IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 2 mg
Presentation Biotin
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rat IgG, IgA and IgM whole molecules.
Application Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/150,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/10,000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Bayer & Wilchek, Methods in Enzymology 184: 138-160 (1990)
Storage Store vial at 2-8°C prior to restoration.
Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below.
Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Goat IgG molecule
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG, IgA and IgM.
This reagent is suitable for the detection of all rat immunoglobulin subclasses, isotypes and chain combinations.
Species:
Rat.
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