R1377HRP Rat IgA + IgG + IgM (H+L chain) antibody

Search for all "Rat IgA + IgG + IgM"

2 mg / €310.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Rat IgA + IgG + IgM

Properties: (H+L chain)
Presentation: HRP
Product is tested for Paraffin Sections, Frozen Sections, Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Rat IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 2 mg
Presentation HRP
Applications C, E, P, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Rat IgG, IgA, IgM whole molecules
Application ELISA: 1/100,000.
Western blot: 1/500-1/3,000.
Immunohistochemistry: 1/500-1/1,500.
Background Rat IgG IgA IgM (H&L) Antibody detects immunoglobulin G, A, and M from Rat. Immunoglobulin G binds to antigens and can neutralize or opsinize targts, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunogobulin molecules linked through their F(c) domain by the J chain.
Caution Do Not Add Sodium Azide
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981 (Conjugation).
  2. Janeway CA, Jr. et al. (2001). Immunobiology. (5th ed.)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Horseradish Peroxidase. 
Conjugation Chemistry: Periodate
Immunoaffinity Chromatography
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
0.01% (w/v) Thimerosal
10 mg/ml BSA (IgG and Protease free)
Lyophilized purified IgG fraction
Restore with 1.0 ml of deionized water (or equivalent).
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Rat antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations.
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