discontinued

NB120-6518 Rat IgG F(ab)2 Fragment antibody

Search for all "Rat IgG F(ab)2 Fragment"

0.25 mg / €250.00

NB120-6518

Product Description for Rat IgG F(ab)2 Fragment

Presentation: AP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Rat IgG F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Presentation AP
Reactivity Rt
Applications E, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Rat IgG F(ab)2 fragment
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/5000 - 1/50000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/5000 to 1/50000 of the reconstitution concentration is suggested for this product.Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/5000 - 1/50000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/5000 to 1/50000 of the reconstitution concentration is suggested for this product.Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA, immunohistochemistry, Western Blot
Concentration 0.6 mg/ml
General Readings General / background references:Avrameas S & Ternynck T Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8:1175-9 (1971). PubMed PMID: 5150003
Storage Store at 4C. Do not freeze.
Format
Label:
Alkaline Phosphatase
AP
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG F(ab)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat Fc or Bovine, Horse, and Human Seru????
Species:
Rat
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