discontinued

NB120-6256 Rat IgG F(c) F(ab)2 Fragment antibody

Search for all "Rat IgG F(c) F(ab)2 Fragment"

0.25 mg / €230.00

NB120-6256

Product Description for Rat IgG F(c) F(ab)2 Fragment

Presentation: Biotin
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for Rat IgG F(c) F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Synonyms Rat Immunoglobulin G
Presentation Biotin
Reactivity Rt
Applications E, ICC/IF, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Rat IgG F(c) fragment
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/15000 - 1/90000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/15000 to 1/90000 of the reconstitution concentration is suggested for this product. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/15000 - 1/90000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/15000 to 1/90000 of the reconstitution concentration is suggested for this product. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., ELISA, immunohistochemistry, immunofluorescence, Western Blot
Concentration 1 mg/ml
General Readings General / background references:Bayer EA & Wilchek M Protein biotinylation. Methods Enzymol 184:138-60 (1990). PubMed PMID: 2388567
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
Biotin
Purification:
Immunogen affinity purified
Buffer System:
0.01% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab')2 or Bovine, Horse and Human Seru????
Species:
Rat
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