discontinued

NB120-6257 Rat IgG F(c) F(ab)2 Fragment antibody

Search for all "Rat IgG F(c) F(ab)2 Fragment"

0.25 mg / €250.00

NB120-6257

Product Description for Rat IgG F(c) F(ab)2 Fragment

Presentation: HRP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Rat IgG F(c) F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Synonyms Rat Immunoglobulin G
Presentation HRP
Reactivity Rt
Applications E, P, WB
Clonality Polyclonal
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Rat IgG F(c) whole molecule
Add. information Dot: Use at an assay dependent dilution. Electron Microscopy: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/20000 - 1/200000. IHC: Use at a dilution of 1/500 - 1/5000. WB: Use at a dilution of 1/20000 - 1/200000. This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/200000 of the reconstitution concentration is suggested for this product for ELISA and Western blots and a 1/500 to 1/5000 for immunohistochemistry on tissue sections. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. Electron Microscopy: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/20000 - 1/200000. IHC: Use at a dilution of 1/500 - 1/5000. WB: Use at a dilution of 1/20000 - 1/200000. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/200000 of the reconstitution concentration is suggested for this product for ELISA and Western blots and a 1/500 to 1/5000 for immunohistochemistry on tissue sections. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., ELISA , immunohistochemistry , immunofluorescence , Western Blot ,
Concentration 1 mg/ml
General Readings General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). PubMed PMID: 6172519
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
HRP
Purification:
Immunogen affinity purified
Buffer System:
0.01% Gentamicin Sulfate
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab?)2 or Bovine, Horse, Human Ser????
Species:
Rat
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