discontinued

NB120-6120 Rat IgG (H&L) F(ab)2 Fragment antibody

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0.25 mg / €250.00

NB120-6120

Product Description for Rat IgG (H&L) F(ab)2 Fragment

Presentation: HRP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Rat IgG (H&L) F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Presentation HRP
Reactivity Rt
Applications E, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Rat IgG whole molecule
Add. information Dot: Use at an assay dependent dilution. Electron Microscopy: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/4000 - 1/16000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4000 to 1/16000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. Electron Microscopy: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/4000 - 1/16000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4000 to 1/16000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., ELISA 1:10,000 - 1:50,000, immunohistochemistry 1:500 - 1:2,500, immunofluorescence , Western Blot 1:1,000 - 1:5,000
Concentration 0.5 mg/ml
General Readings General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). PubMed PMID: 6172519
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
HRP
Purification:
Immunogen affinity purified
Buffer System:
0.01% Gentamicin Sulfate
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Pro???
Species:
Rat
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