discontinued

NB120-6251 Rat IgG (H&L) F(ab)2 Fragment antibody

Search for all "Rat IgG (H&L) F(ab)2 Fragment"

0.25 mg / €250.00

NB120-6251

Product Description for Rat IgG (H&L) F(ab)2 Fragment

Presentation: AP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Rat IgG (H&L) F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Synonyms Rat Immunoglobulin G
Presentation AP
Reactivity Rt
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Rat IgG whole molecule
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/1000 - 1/4000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/4000 of the reconstitution concentration is suggested for this product.Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/1000 - 1/4000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/4000 of the reconstitution concentration is suggested for this product.Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA, immunohistochemistry, Western Blot
Concentration 1 mg/ml
General Readings General / background references:Avrameas S & Ternynck T Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8:1175-9 (1971). PubMed PMID: 5150003
Storage Store at 4C. Do not freeze.
Format
Label:
Alkaline Phosphatase
AP
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c), or Bovine, Horse, Human, Mouse, Rabbit and ????
Species:
Rat
  • LinkedIn