R1371F Rat IgG (H+L chain) antibody

Search for all "Rat IgG"

2 mg / €200.00
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R1371F

Product Description for Rat IgG

Properties: (H+L chain)
Presentation: FITC

Properties for Rat IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Rat Immunoglobulin G
Presentation FITC
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rat IgG whole molecule.
Application Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
Immunofluorescence: 1/1,000-1/5,000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate (Molecular Weight 390 daltons)
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
3.1 moles FITC per mole of Rabbit IgG.
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified IgG fraction.
Reconstitution:
Restore with 1.0 ml of deionized water or equivalent.
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgG and Rat Serum.
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