R1371HRP Rat IgG (H+L chain) antibody

Search for all "Rat IgG"

2 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1371HRP

Product Description for Rat IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Frozen Sections, Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Rat IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Rat Immunoglobulin G
Presentation HRP
Applications C, E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rat IgG whole molecule
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/170,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,500.
Caution Do NOT add Sodium Azide!
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term. 
Centrifuge product if not completely clear after standing at RT.   
Dilute only prior to immediate use. 
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity Chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin Sulfate
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum.
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