discontinued

R1415R Rat IgG F(ab)2 specific (adsorbed F(ab)2 Fragment) antibody

Search for all "Rat IgG"

R1415R

Product Description for Rat IgG

Properties: F(ab)2 specific (adsorbed F(ab)2 Fragment)
Presentation: PE

Properties for Rat IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 ml
Synonyms Rat Immunoglobulin G
Presentation PE
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rat IgG F(ab')2 fragment
Application Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Concentration Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
Storage Store the antibody at 2-8°C prior to opening.
DO NOT FREEZE!
Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
This product is stable at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Format
Label:
R-Phycoerythrin ( isolated from a culture of red algae. Subunit structure - (αβ)6γ Apparent molecular weight - 240 kDa (alpha 20 kDa, beta 20 kDa, gamma 30 kDa)
PE
Absorption emission:
490 nm, 545 nm and 565 nm / 580 nm
Purification:
Immunoaffinity chromatography.
Buffer System:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6 with 0.05% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
State:
Lyophilized F(ab')2 fragments.
Reconstitution:
Restore with 1.0 ml of deionized water.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
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