R1439C5 Rat IgG (H+L chain, adsorbed) antibody

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Product Description for Rat IgG

Properties: (H+L chain, adsorbed)
Presentation: Cy5

Properties for Rat IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Rat Immunoglobulin G
Presentation Cy5
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Rat IgG, whole molecule.
Application Suitable for Immunomicroscopy (1/100-1/1,000) and Flow cytometry (1/200-1/400) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Minimum ELISA Endpoint Dilution: 1/10,000.
Actual: 1/110,000.
Disposition: PASS.
Concentration 1.1 mg/ml (by UV absorbance at 280 nm)
Storage Store vial at 2-8° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
(Molecular weight 975 daltons)Color: Far-Red
Absorption emission:
650 nm / 667 nm
Molar radio:
17.9 moles Cy5 per mole of Goat IgG
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
Lyophilized purified IgG fraction.
Restore with 1.0 ml of deionized water or equivalent.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
Only minimum cross reactivity was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Sheep and Human Serum Proteins.
Actual Cross Reactivities:
Bovine 0.4%, Chicken 0.01%, Goat 0.4%, Guinea Pig 0.01%, Hamster 0.01%, Horse 0.4%, Mouse 0.01%, Rabbit 0.8%, Sheep 0.4%, Human 0.01%
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