AP21493FC-N Rat IgG2ab (subclass specific) antibody

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1 ml / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP21493FC-N

Product Description for Rat IgG2ab

Properties: (subclass specific)
Presentation: FITC
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Rat IgG2ab

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Rat Immunoglobulin G2ab
Presentation FITC
Reactivity Rt
Applications C, E, ICC/IF, ID, IP
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Pools of highly purified homogenous IgG2a and IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required.
Application Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/20 - 1/80.
Concentration 10,0 mg/ml
Storage Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate
FITC
Absorption emission:
492 nm / 515 nm
Molar radio:
Fluorochrome/IgG: ~1.6
Purification:
Immunoaffinity Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore by adding 0.5 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Further tested on the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells. When used on vital peripheral blood cells this immunoconjugate does not react with Fc receptors present on cell surface membranes.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
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