AP21493SU-N Rat IgG2ab (subclass specific) antibody

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1 ml / €250.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP21493SU-N

Product Description for Rat IgG2ab

Properties: (subclass specific)
Presentation: Serum
Product is tested for Immunoprecipitation.

Properties for Rat IgG2ab

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Rat Immunoglobulin G2ab
Presentation Serum
Reactivity Rt
Applications IP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Pools of purified homogenous IgG2a and IgG2b isolated from Rat serum. 
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Immunoprecipitation.
In precipitating techniques as Immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a and/or IgG2b in Rat serum and other body fluids or to determine its concentration.
To prepare an immunoadsorbent for the purification of Rat IgG2a/IgG2b from serum or plasma.
Recommended Working Dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less then 1/64 when tested against pooled normal Rat serum in agar block immunodiffusion titration.
Concentration Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
None
State:
Lyophilized (Delipidated and heat inactivated) stable whole antiserum
Serum
Reconstitution:
Restore by adding 1.0 ml sterile distilled water.
Dilutions may be prepared by adding PBS, pH 7.2
Species Reactivity
Species reactivity (tested):
Rat.
Specificity
Specificity:
The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
Cross-reactivity: This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.
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