AP33082BT-N Sheep IgG (H+L chain) antibody

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1 ml / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP33082BT-N

Product Description for Sheep IgG

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Paraffin Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Enzyme Immunoassay, Dot blot.

Properties for Sheep IgG

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Sheep Immunoglobulin G
Presentation Biotin
Reactivity Sh
Applications Dot, E, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Not required
Application ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
N-Hydroxysuccinimidobiotin
Biotin
Molar radio:
Biotin/IgG ~4.2
Purification:
Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2
Preservatives:
None, as it may interfere with the antibody activity.
Stabilizers:
None
State:
Lyophilized purified hyperimmune IgG fraction
Reconstitution:
Restore with 1 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Sheep.
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