AP33082SU-N Sheep IgG (H+L chain) antibody

Search for all "Sheep IgG"

1 ml / €190.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Sheep IgG

Properties: (H+L chain)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Sheep IgG

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Sheep Immunoglobulin G
Presentation Serum
Reactivity Sh
Applications ID, IP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Not required
Application Immunoprecipitation. 
Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations.
Recommended Working Dilutions:
Immunoelectrophoresis: use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Concentration Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
None, as it may interfere with the antibody activity.
Delipidated, heat inactivated lyophilized stable whole serum
Restore with 1 ml sterile distilled water.
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine: ++, Cat: ±, Chicken: -, Dog: ±, Goat: ++, Guinea pig: ±, Hamster: ±, Horse: +, Human: +, Mouse: +, Monkey: +, Pigeon: -, Rat: +, Swine: +, Turkey: -
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