R1433R Sheep IgG (H+L chain), adsorbed F(ab')2 Fragment antibody

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0.5 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1433R

Product Description for Sheep IgG

Properties: (H+L chain), adsorbed F(ab')2 Fragment
Presentation: PE

Properties for Sheep IgG

Product Category Secondary Antibodies
Target Category
Quantity 0.5 mg
Synonyms Sheep Immunoglobulin G
Presentation PE
Clonality Polyclonal
Host Donkey
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Sheep IgG whole molecule
Application Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Storage Store the antibody (undiluted) at 2-8°C prior to opening.
DO NOT FREEZE!.
Store reagent in the dark. Use subdued lighting during handling and incubation
of cells prior to analysis.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Format
Label:
R-Phycoerythrin ( (Molecular Weight 240,000 daltons) from seaweed. Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound)
PE
Absorption emission:
490 nm, 545 nm and 565 nm / 580 nm
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride; pH 7.2 with 0.02% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free, as stabilizer.
State:
Lyophilized F(ab')2 fragments.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum.
No reaction was observed against anti-Pepsin, anti-Donkey IgG Fc, or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins.
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