R1456AP Sheep IgG (H+L chain) antibody

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1 mg / €270.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1456AP

Product Description for Sheep IgG

Properties: (H+L chain)
Presentation: AP

Properties for Sheep IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Sheep Immunoglobulin G
Presentation AP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Sheep IgG whole molecule
Application Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/25,000.
Western Blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)
Storage Store the antibody undiluted at 2-8°C before opening.
DO NOT FREEZE!
Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (MW. 140,000 daltons)
AP
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
State:
Liquid (sterile filtered) purified Ig fraction.
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum.
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