R1456B Sheep IgG (H+L chain) antibody

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2 mg / €220.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1456B

Product Description for Sheep IgG

Properties: (H+L chain)
Presentation: Biotin

Properties for Sheep IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Sheep Immunoglobulin G
Presentation Biotin
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Sheep IgG whole molecule
Application Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 is suggested for this product.
Concentration 2.0 mg/ml ( by UV absorbance at 280 nm)
General Readings Bayer & Wilchek: Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. Restore with deionized water. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule
Purification:
Immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% Sodium Azide as preservative
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG, and Sheep Serum.
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