R1456F Sheep IgG (H+L chain) antibody

Search for all "Sheep IgG"

2 mg / €200.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1456F

Product Description for Sheep IgG

Properties: (H+L chain)
Presentation: FITC

Properties for Sheep IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Sheep Immunoglobulin G
Presentation FITC
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Sheep IgG whole molecule.
Application Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Concentration 2.2 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. The and Feltkamp, Immunology 18; 865, 1970 (Conjugation).
Storage Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate (Molecular Weight 390 daltons)
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
3.3 moles FITC per mole of Rabbit IgG
Purification:
Immunoaffinity Chromatography
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG and Sheep Serum.
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