AP33084BT-N Sheep IgM (H+L chain) antibody

Search for all "Sheep IgM"

1 ml / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP33084BT-N

Product Description for Sheep IgM

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Paraffin Sections, Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Dot blot, Enzyme Immunoassay.

Properties for Sheep IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Sheep Immunoglobulin M
Presentation Biotin
Reactivity Sh
Applications Dot, E, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other serum proteins. 
Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
Application ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
N-Hydroxysuccinimidobiotin
Biotin
Molar radio:
Biotin/IgG ~5.7
Purification:
Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2
Preservatives:
None, as it may interfere with the antibody activity.
Stabilizers:
None
State:
Lyophilized purified hyperimmune IgG fraction
Reconstitution:
Restore with 1 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgM molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Sheep.
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