Product Description for Sheep IgM
Product is tested for Immunodiffusion, Immunoprecipitation.
Properties for Sheep IgM
|Product Category||Secondary Antibodies|
|Synonyms||Sheep Immunoglobulin M|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Polyclonal Antibodies (de)|
|Add. information||Adsorption: Not required.|
Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgM and IgG antibodies, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations.
Recommended Working Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
|Concentration||Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added.|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.