NB900-62085 10X Tris-EDTA Buffer pH 9.0

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Properties for 10X Tris-EDTA Buffer pH 9.0

Product Category Buffers & Chromogens
Quantity 0.5 l
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Dilute one part buffer in nine parts distilled water.
Application , Dilute one part buffer in nine parts distilled water.
Background In order to perform immunostaining, the tissue specimens should be fixed in appropriate fixative. The purpose of such fixative is to conserve the tissue from autolysis, to preserve tissue structures and to immobilize antigens. However, this requires harsh treatment of the antigens. As a result, antigens undergo chemical alteration of their primary, secondary and tertiary structures. Because of changes in the protein containing epitopes or in neighboring proteins, antigenic sites may be masked. In past, enzymatic treatment with proteolytic enzymes i.e. pepsin, trypsin or pronase has been performed to regain the masked antigens. Shi et al. (1991) have reported that the treatment of the tissue section with heavy metal solution in a microwave can regain the masked antigens significantly. However, heavy metals in the solution increase the risk of exposure of lab personals to lead. To solve this problem, we have developed a new antigen unmasking solution in tablet form, which is free from heavy metals. Use of this antigen unmasker can prevent the risk of unnecessary exposure to the lab personal and also resolve the handling and disposal problems.
Storage Store at room temperature.
Buffer System:
No Preservative
10X Tris-EDTA Buffer for Heat Induced Epitope Recovery, pH 9.0

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