discontinued

NB900-81105 10X Universal ELISA Wash Buffer (WB1)

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100 ml / €140.00

NB900-81105

Properties for 10X Universal ELISA Wash Buffer (WB1)

Product Category Buffers & Chromogens
Quantity 100 ml
Synonyms ELISA Universal buffer, ELISA buffer, ELISA diluents, Universal Wash diluent
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Add 1 part WB1 to 9 parts deionized water and mix for 15 minutes. To wash microtiter plates, 1X wash buffer may be dispensed through a squirt bottle, a plate washer, a multi-channel pipette, or through an automated system. If washing by hand, be sure to fill the wells all the way up with 1X wash buffer (about 400uL/well). Then aspirate or dump out the buffer and repeat for a total of 2-4 washes. After the final dump or aspiration, tamp the plate on paper towels to remove any excess liquid. We do not recommend submerging the entire plates in a bath as many of the wells will become contaminated, decreasing the reliability of the assay. When washing a plate during the coating process, we recommend 2-3 washes between steps. When washing the plate as part of an ELISA procedure, we recommend 2-4 washes between steps.
Application ELISA, Add 1 part WB1 to 9 parts deionized water and mix for 15 minutes. To wash microtiter plates, 1X wash buffer may be dispensed through a squirt bottle, a plate washer, a multi-channel pipette, or through an automated system. If washing by hand, be sure to fill the wells all the way up with 1X wash buffer (about 400uL/well). Then aspirate or dump out the buffer and repeat for a total of 2-4 washes. After the final dump or aspiration, tamp the plate on paper towels to remove any excess liquid. We do not recommend submerging the entire plates in a bath as many of the wells will become contaminated, decreasing the reliability of the assay. When washing a plate during the coating process, we recommend 2-3 washes between steps. When washing the plate as part of an ELISA procedure, we recommend 2-4 washes between steps., ELISA
Background 5x Universal ELISA Wash Buffer (WB1) WB1 is used to rinse microtiter plates during the coating process and between reagent addition steps of an ELISA. WB1 is a universal ELISA wash buffer: it may be used with antibody-sandwich ELISAs and antigen-down ELISAs. WB1 is an optimal formulation of pH stabilizers, salts, and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background noise and increasing the specific signal. WB1 is compatible with all routinely used conjugate components such as HRP, AP, and avidin, among others. - Can be used with all ELISA formats and conjugate components. - Reduces nonspecific background noise. - pH 7.2 at 1X, ionic strength.
Storage Store at 4C. Do not freeze.
Format
Purification:
protein A purified
Buffer System:
Clear liquid, supplied as a 10X concentrate. Concentrate may crystallize when refrigerated. If crys
Purity:
protein A purified
Specificity
Specificity:
10X Universal ELISA Wash Buffer (WB1)

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