discontinued

NB900-81011 5X Antibody Plate Coating Buffer 1 (CB1)

Search for all "5X Antibody Plate Coating Buffer 1 (CB1)"

100 ml / €140.00

NB900-81011

Properties for 5X Antibody Plate Coating Buffer 1 (CB1)

Product Category Buffers & Chromogens
Quantity 100 ml
Synonyms Coating buffer, ELISA coating buffer, ELISA diluents
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Add 1 part CB1 to 4 parts deionized water and mix for 5-15 minutes.
Add the antibody to be coated to the 1X buffer and mix for 5-15 minutes.
Coat the plate by pipetting the solution into the wells of the ELISA plate.
Application ELISA, Add 1 part CB1 to 4 parts deionized water and mix for 5-15 minutes. Add the antibody to be coated to the 1X buffer and mix for 5-15 minutes. Coat the plate by pipetting the solution into the wells of the ELISA plate., ELISA ,
Background 5x Antibody Plate Coating Buffer (CB1) maximizes the adsorption of antibodies onto polystyrene plates for use in Antibody-sandwich ELISAs. During the adsorption process, CB1 stabilizes the coating antibody maintaining its three-dimensional structure. By stabilizing the adsorbed antibody's tertiary structure, the antigen recognition regions are preserved. This allows for greater binding reactivity with the target antigen thereby enhancing the specific signal. By generating a higher specific signal, a lower concentration of coating antibody may be needed when this buffer is used, thereby saving valuable reagents. - Stabilizes the tertiary structure of antibodies to enhance their reactivity. - Buffered reagent to coat antibodies onto polystyrene microtiter ELISA plates. - Enhances adsorption of antibodies onto polystyrene microtiter ELISA plates. - pH 8.4 at 1X, ionic strength. - Plates can be coated at room temperature. - Retards bacterial growth. - Can be used at 50uL - 300uL per well at 1X.
Storage Store at 4C. Do not freeze.
Specificity
Specificity:
5X Antibody Plate Coating Buffer 1 (CB1)

Accessory Products

  • LinkedIn