discontinued

NB900-81099 IgM-Reducing Assay Diluent (AD2)

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100 ml / €150.00

NB900-81099

Properties for IgM-Reducing Assay Diluent (AD2)

Product Category Buffers & Chromogens
Quantity 100 ml
Synonyms Assay buffer, Assay diluent, ELISA Assay buffer, ELISA diluents, IgM diluent
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Once the capture antibody has been coated onto the plate and the plate has been blocked, pipet 50-100uL of AD2 into the wells of the ELISA plate prior to adding the standard or test sample to each well.
Application ELISA, Once the capture antibody has been coated onto the plate and the plate has been blocked, pipet 50-100uL of AD2 into the wells of the ELISA plate prior to adding the standard or test sample to each well., ELISA ,
Background IgM-Reducing Assay Diluent (AD2) is formulated for use with serum and plasma samples, and has been developed for use in all antibody sandwich ELISA formats. AD2 contains a mammalian protein additive (based on normal goat serum) to reduce non-specific interactions between the sample matrix and the plate surface. Assay diluent is important for equalizing any differences between the sample matrix and the diluent used to generate the standard curve in the ELISA. AD2 contains proprietary additives designed to inhibit complement and thrombin activity present in serum and plasma samples and is designed to reduce IgM-mediated conjugate bridging interference that can cause substantial background noise. - Can be used for antibody-sandwich ELISAs. - Contains a mammalian protein blocking agent based on NGS to reduce nonspecific interactions between the sample matrix proteins and the plate surface, translating into lower background noise. - Contains additives to inhibit complement and thrombin activity in serum and plasma samples. - Formulated at 1X, ready to use. - pH 7.4 at 1X, ionic strength. - Can be used at 50uL - 100uL per well at 1X.
Storage Store at 4C. Do not freeze.
Format
Buffer System:
Clear liquid, supplied as a 1X concentrate.
Specificity
Specificity:
IgM-Reducing Assay Diluent (AD2)

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