discontinued

NB900-81054 Neptune Block (BB2)

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100 ml / €410.00

NB900-81054

Properties for Neptune Block (BB2)

Product Category Buffers & Chromogens
Quantity 100 ml
Synonyms Blocking buffer, ELISA Blocking buffer, ELISA diluents, Neptune Block
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Once antibody or antigen has been coated onto the plate and incubated, aspirate the coating solution from the plate. Block the plate by pipetting 300-400uL of BB2 into the wells of the ELISA plate (use the same volume or more of BB2 as was used for the coating solution). Incubate 1-24 hours, according to your protocol.
Application ELISA, Once antibody or antigen has been coated onto the plate and incubated, aspirate the coating solution from the plate. Block the plate by pipetting 300-400uL of BB2 into the wells of the ELISA plate (use the same volume or more of BB2 as was used for the coating solution). Incubate 1-24 hours, according to your protocol., ELISA ,
Background Neptune Block wth Nonmammalian-Based Blockers (BB2) contains a proprietary fish extract plus molecular stabilizers that provide a high degree of blocking efficiency. Neptune Block is designed for antigen-down ELISA formats, and for sandwich assays with high background problems. BB2 is a heterogeneous mixture of small molecules capable of blocking both the nonspecific binding sites of the adsorbed protein, as well as the unoccupied regions of the polystyrene plate that are not sterically accessible to larger traditional mammalian blocking reagents; thereby reducing background noise. The small size of this unique blocker results in minimal steric hindrance to key epitope regions of target coated proteins, or entirely masking small coated peptides, thus enhancing the generation of a specific signal. Since Neptune Block is a nonmammalian protein blocking agent, it is antigenically foreign to most mammalian immune systems. In antigen-down ELISA formats, this reduces the possibility of false-positives generated from endogenous antibodies in the sample reacting with plate blocking proteins. Neptune Block is particularly useful when working with human, swine, and bovine serum matrices, as there is little interaction with the blocking molecules resulting in lower backgrounds. Neptune Block also contains an antimicrobial agent for room temperature blocking and, when dried, long-term storage at 2-8C. - For antigen-down ELISAs. - For antibody-sandwich ELISAs with high background problems. - Particularly useful for human, swine, and bovine sera. - Contains a proprietary fish extract that is antigentically foreign to most mammalian immune systems. - Contains a heterogeneous mixture of small molecules - Maintains the water of hydration of dried antigen or antibody coat proteins. - Minimizes nonspecific binding interactions during the assay process. - Formulated at 1X, ready to use. - pH 7.4 at 1X, ionic strength - Plates can be coated at room temperature. - Contains anti-bacterial agents to extend the shelf-life of coated plates. - Can be used at 300uL - 400uL per well at 1X.
Storage Store at 4C. Do not freeze.
Format
Buffer System:
Pale Yellow liquid, supplied at 1X.
Specificity
Specificity:
Neptune Block (BB2)

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