discontinued

NB900-81103 Plasma Serum Diluent

Search for all "Plasma Serum Diluent"

100 ml / €200.00

NB900-81103

Properties for Plasma Serum Diluent

Product Category Buffers & Chromogens
Quantity 100 ml
Synonyms Antigen down diluent, ELISA Plasma buffer, ELISA diluents, Plasma buffer, Plasma diluent
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information When diluting test samples; note the dilution factor for future data analysis. For example, if you diluted your sample 1:2 (250uL serum sample into 250uL sample diluent) and this yielded an OD of 0.437, then when read off the curve the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL. This indicates that the original starting sample had 400pg/mL of analyte present.
Application ELISA, When diluting test samples; note the dilution factor for future data analysis. For example, if you diluted your sample 1:2 (250uL serum sample into 250uL sample diluent) and this yielded an OD of 0.437, then when read off the curve the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL. This indicates that the original starting sample had 400pg/mL of analyte present., ELISA ,
Background Plasma Sample Diluent (SD2) is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This diluent contains proprietary additives in a buffered BSA protein base that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interactions. Additional additives have been included to inhibit thrombin (clotting) and complement activity during the plasma sample incubation period. SD2 also contains an antimicrobial agent to provide a solution free of bacterial contamination. - Can be used for antigen-down ELISAs to detect antigen-specific immunoglobin in plasma. - May be used in sandwich ELISA formats to dilute samples and antigen standards. - Contains additives to reduce nonspecific binding interactions. - Contains an antimicrobial agent to provide a solution free of bacterial contamination. - Formulated at 1X, ready to use. - pH 7.4 at 1X, ionic strength.
Storage Store at 4C. Do not freeze.
Format
Buffer System:
Clear liquid, supplied at 1X concentrate.
Specificity
Specificity:
Plasma Serum Diluent (SD2)

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