discontinued

NB900-81071 SynBlock (BB3)

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100 ml / €150.00

NB900-81071

Properties for SynBlock (BB3)

Product Category Buffers & Chromogens
Quantity 100 ml
Synonyms Blocking buffer, ELISA Blocking buffer, ELISA diluents, SynBlock
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Once antibody or antigen has been coated onto the plate and incubated, aspirate the coating solution from the plate. Block the plate by pipetting 300-400uL of BB3 into the wells of the ELISA plate (use the same volume or more of BB3 as was used for the coating solution). Incubate 1-24 hours, according to your protocol.
Application ELISA, Once antibody or antigen has been coated onto the plate and incubated, aspirate the coating solution from the plate. Block the plate by pipetting 300-400uL of BB3 into the wells of the ELISA plate (use the same volume or more of BB3 as was used for the coating solution). Incubate 1-24 hours, according to your protocol. , ELISA ,
Background SynBlock with Tween and Synthetic Blockers is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Because SynBlock contains inert nonreactive blockers, it reduces the amount of nonspecific binding of enzyme labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives. SynBlock also contains an antimicrobial agent for room temperature blocking on the benchtop and enables plates to be stored, when dried, at 2-8C for up to 18 months. - For antigen-down ELISAs. - For antibody-sandwich ELISAs. - Contains inert, nonreactive blockers to reduce nonspecific binding of enzyme labeled conjugates to blocked plate surfaces. - Maintains the water of hydration of dried antigen or antibody coat proteins. - Minimizes nonspecific binding interactions during the assay process. - Formulated at 1X, ready to use. - pH 7.4 at 1X, ionic strength - Plates can be coated at room temperature - Contains anti-bacterial agents to extend the shelf-life of coated plates. - Can be used at 300uL - 400uL per well at 1X.
Storage Store at 4C. Do not freeze.
Format
Buffer System:
Clear liquid, supplied at 1X.
Specificity
Specificity:
SynBlock (BB3)

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