LC810220 SOAT1-knockout Cell lysate

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0.1 mg / €495.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

LC810220

Properties for SOAT1-knockout Cell lysate

Product Category Lysates (Knockout)
Target Category
Quantity 0.1 mg
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P35610
Add. information
SOAT1-knockout Cell lysate from HeLa cell ine, one vial of parental cell line lysate included as control
Component:
1 vial of 100 ug KO cell lysate (lyophilized)
1 vial of 100ug parental cell lysate (lyophilized)
Application Western Blot.
Background Parental cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The KO cell lysates are the cell homogenate in RIPA buffer made from the KO cell lines. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control. To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Storage Upon receiving, store the sample at -20°C.
Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer and will be stable at -20°C for 3 months.
Format
Reconstitution:
To use as WB control, spin down briefly and resuspend in 20ul 2X SDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT).
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