Antibodies to 5-hydroxytryptamine (Serotonin) Receptors - FocusOn 042

Introduction: 5-hydroxytryptamine (5-HT), also known as Serotonin, is probably unique among the monoamines in that its effects are subserved by as many as thirteen distinct heptahelical, G-protein-coupled receptors (GPCRs) and one (presumably a family of) ligand-gated ion channel(s). These receptors are divided into seven distinct classes [5-HT(1) to 5-HT(7)] largely on the basis of their structural and operational characteristics.

Whilst this degree of physical diversity clearly underscores the physiological importance of serotonin, evidence for an even greater degree of operational diversity continues to emerge. The challenge for modern 5-HT research has therefore been to define more precisely the properties of the systems that make this incredible diversity possible. Much progress in this regard has been made during the last decade with the realisation that serotonin is possibly the least conservative monoamine transmitter and the cloning of its many receptors. Coupled with the actions of an extremely avid and efficient reuptake system, this array of receptor subtypes provides almost limitless signalling capabilities to the extent that one might even question the need for other transmitter systems. However, the complexity of the system appears endless, since posttranslational modifications, such as alternate splicing and RNA editing, increase the number of proteins, oligomerisation and heteromerisation increase the number of complexes, and multiple G-protein suggest receptor trafficking, allowing phenotypic switching and crosstalk within and possibly between receptor families. Whether all these possibilities are used in vivo under physiological or pathological conditions remains to be firmly established.

Antibody Tools for the Specific Detection of 5-HT Receptors

For specific detection of the 13 serotonin receptor subtypes a unique antibody panel is available from Acris Antibodies. All antibodies were made using receptor specific peptides for immunisation. Every single antibody was tested thoroughly for the applications listed in the table below. A picture gallery at the end of this article shows staining results achieved using these products.



Immunohistochemical staining of Vascular smooth muscle using anti-HTR1B antibody SP4041P.
Immunohistochemical staining of Vascular smooth muscle using anti-HTR1B antibody Cat.-No. SP4041P
Immunohistochemical staining of Kidney (Renal tubules) using anti- HTR1F antibody SP4034P
Immunohistochemical staining of Kidney (Renal tubules) using anti- HTR1F antibody Cat.-No. SP4034P
Staining of 5-HT (Serotonin) 2A Receptor Antibody 24288
Staining of 5-HT (Serotonin) 2A Receptor Antibody Cat.-No. 24288
 Immunohistochemical staining of Brain (Neurons and Glia) using anti-HTR2B antibody SP4046P.
Immunohistochemical staining of Brain (Neurons and Glia) using anti-HTR2B antibody Cat.-No. SP4046P
Immunohistochemical staining of brain (neurons and glia) using anti-5-HT2b receptor antibody SP4047P
Immunohistochemical staining of brain (neurons and glia) using anti-5-HT2b receptor antibody Cat.-No. SP4047P
 Immunohistochemical staining of brain (neurons and glia) using antibody SP4048P (Formalin-Fixed Paraffin Embedded).
Immunohistochemical staining of brain (neurons and glia) using antibody (Formalin-Fixed Paraffin Embedded). Cat.-No. SP4048P
Immunohistochemical staining of Brain (Neuron) using anti- HTR6 antibody SP4049P
Immunohistochemical staining of Brain (Neuron) using anti- HTR6 antibody Cat.-No. SP4049P
Immunohistochemical staining of Brain (Neurons and Astrocytes) using anti- HTR7 antibody SP4050P
Immunohistochemical staining of Brain (Neurons and Astrocytes) using anti- HTR7 antibody Cat.-No. SP4050P
Immunohistochemical staining of Brain (neurons and glia) using anti- HTR7 antibody SP4051P
Immunohistochemical staining of Brain (neurons and glia) using anti- HTR7 antibody Cat.-No. SP4051P

Antibody Panel to Serotonin Receptors

Serotonin receptor (5-hydroxytryptamine receptor / 5-HT) : HTR1A (5-HT1A Receptor, 5-HT1AR), HTR1B, HTR1D, HTR1E, HTR1F, HTR2A, HTR2B, HTR2C, HTR3A, HTR3B, HTR4, HTR5A, HTR6, HTR7, and HTRA2



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Serotonin receptor 1A (HTR1A) antibody

Immunohistochemistry (IHC) analyzes of SR-1A antibody (Cat.-No.: AP06769PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Serotonin receptor 1A (HTR1A) antibody

Serotonin receptor 1A (HTR1A) Rabbit Serum Hu, Ms, Rt C, ICC/IF
0.1 ml / €490.00
  ImmunoStar Inc.

Serotonin receptor 1E (HTR1E) (3rd Cytopl. Dom.) antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Elephant, Eq, GP, Hu, Mky, Op, Prim P
50 µg / €470.00
  Acris Antibodies GmbH

Serotonin receptor 2A (HTR2A) antibody

Staining of 5-HT (Serotonin) 2A Receptor Antibody 24288 Rabbit Serum Ms, Rb, Rt C, ICC/IF
0.1 ml / €490.00
  ImmunoStar Inc.

Serotonin receptor 2C (HTR2C) antibody

Staining with 5-HT (Serotonin) 2C Receptor Antibody (24505) Rabbit Serum Hu, Ms, Rt C, ICC/IF
0.1 ml / €490.00
  ImmunoStar Inc.

Serotonin receptor 3A (HTR3A) antibody

Anti-5HT3A receptor antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit IgG Aff - Purified Hu, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Serotonin receptor 3A (HTR3A) (428-439) antibody

Immunohistochemistry Image: Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Hu, Prim E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Serotonin receptor 3E (HTR3E) (Center) antibody

5HT3E Antibody (Center) (Cat. #AP50002PU-N) western blot analysis in CEM cell line lysates (35 µg/lane).This demonstrates the 5HT3E antibody detected the 5HT3E protein (arrow) Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Serotonin receptor 4 / HTR4 (237-250) antibody

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Can, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Por, Prim, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

Serotonin receptor 4 / HTR4 (N-term) antibody

Western blot analysis of HTR4 Antibody (N-term) Cat.-No AP52130PU-N in MDA-MB453 cell line and mouse liver tissue lysates (35ug/lane). This demonstrates the HTR4 antibody detected the HTR4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Serotonin receptor 5A (HTR5A) (17-34) antibody

Staining with 5-HT (Serotonin) 5A Receptor Antibody (24429) Rabbit Serum Ms, Rt C, ICC/IF
0.1 ml / €490.00
  ImmunoStar Inc.

Serotonin receptor 5B (HTR5B) antibody

Western blot (WB) analysis of SR-5B antibody (Cat.-No.: AP01293PU-N) in extracts from COLO cells. Rabbit Aff - Purified Ms, Rt E, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Serotonin receptor 6 (HTR6) (C-term) antibody

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P
50 µg / €470.00
  Acris Antibodies GmbH

Serotonin receptor 7 (HTR7) (3rd Cytopl. Dom.) antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Can, Eq, GP, Hst, Hu, Mky, Ms, Op, Por, Prim, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

Serotonin receptor 7 (HTR7) (C-term) antibody

Formalin-Fixed Paraffin-Embedded Human Brain cerebellum stained with HTR7 Antibody Cat.-No AP07036PU-N Rabbit Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Op, Por, Prim, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

Serotonin receptor 7 (HTR7) (8-23) antibody

Staining with 5-HT (Serotonin) 7 Receptor Antibody 24430 Rabbit Serum Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €490.00
  ImmunoStar Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Serotonin BSA Conjugate Control

Serotonin
50 µg / €240.00
  ImmunoStar Inc.

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