New Annexin Antibody Panel – Analyzing the Protein Family of Membrane-Managers - FocusOn 110

The Annexins belong to a group of membrane-binding proteins, who share a conserved C-terminal domain, which contains a Ca2+-dependent phospholipids binding site. They differ by their N-terminal domain, which determines the special function of each annexin.

Annexins are predominantly localized at the cytoplasmic site of the plasma membrane. Although their functions are yet not quite clear defined, it is meanwhile known, that some members of the Annexin family are part of Ca2+-dependent signal transduction pathways as well as exocytotic and endocytotic processes. They also play a role in the regulation of cell growth.

Members of the Annexin-Family

Many members of the Annexin family, like Annexin A1, A3, A4 and A5 show a Phospholipase A2 (PLA2) inhibiting activity. Because PLA2 is necessary for the biosynthesis of potent mediators of inflammation, like prostaglandins and leukotriens, these Annexins have antiinflamatory activity. Annexin A5 inhibits besides PLA2 also the Protein Kinase C (PKC).

Annexin A2 functions as autocrine factor, promoting osteoclast formation and bone resorption.
Some Annexins like Annexin A3 and A4 play a role in the regulation of coagulation an show anticoagulating activity. Annexin A4 also interacts with ATP. It is expressed exclusively in epithelial cells.

Some Annexins like Annexin A5 and A7 are able to work as calcium channels.

Other Annexins, like Annexin A6 (wich has high homology to Annexin A1 and A2) and Annexin A7 have membrane fusing functions, as are required for vesicle fusion during exocytosis, f.e.

Annexin A9 contains 4 Annexin repeats and possibly functions as a low affinity receptor for acetylcholin. It is expressed in stratified, squamous skin epithelium.

Annexins are also implicated in some diseases. Sera of patients having different autoimmune diseases f.e., recognize Annexin A11 as antigen.

The functions of Annexin A13 have not been determined, but it is known to be associated with the plasma membrane of undifferenciated, proliferating endothelial cells and differenciated enterocytes of intestinal villi.

Tab. 1: Human Annexins

Name

Synonyms

Gen

theoret. MW

A1

Annexin I, Lipocortin I, Calpactin II, Chromobindin-9, p35, Phospholipase A2 inhibitory protein

ANXA1 (ANX1, LPC1)

~ 39 kD

A2

Annexin II, Lipocortin II, Calpactin I heavy chain, Chromobindin-8, p36, Protein I, Placental anticoagulant protein IV, PAP-IV

ANXA2 ( ANX2, ANX2L4, CAL1H,LPC2D )

~ 38 kD

A3

Annexin III, Lipocortin III, Placental anticoagulant protein III, PAP-III, 35-alpha calcimedin, Inositol 1,2-cyclic phosphate 2-phosphohydrolase

ANXA3 (ANX3)

~ 36 kD

A4

Annexin IV, Lipocortin IV, Endonexin I, Chromobindin-4, Protein II, P32.5, Placental anticoagulant protein II, PAP-II, PP4-X, 35-beta calcimedin, Carbohydrate-binding protein P33/P41, P33/41

ANXA4 (ANX4)

~ 36 kD

A5

Annexin V, Lipocortin V, Endonexin II, Calphobindin I, CBP-I, Placental anticoagulant protein I, PAP-I, PP4, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, Anchorin CII

ANXA5 (ANX5, ENX2, PP4)

~ 36 kD

A6

Annexin VI, Lipocortin VI, P68, P70, Protein III, Chromobindin-20, 67 kDa calelectrin, Calphobindin-II, CPB-II

ANXA6 (ANX6)

~ 76 kD

A7

Annexin VII, Synexin

ANXA7 (ANX7, SNX)

~ 53 kD

A8

Annexin VIII, Vascular anticoagulant-beta, VAC-beta

ANXA8 (ANX8)

~ 37 kD

A9

Annexin-9, Annexin-31, Annexin XXXI, Pemphaxin

ANXA9 (ANX31)

~ 38 kD

A10

Annexin-10, Annexin-14

ANXA10 (ANX14)

~ 37 kD

A11

Annexin XI, Calcyclin-associated annexin 50, CAP-50, 56 kDa autoantigen

ANXA11 (ANX11)

~ 54 kD

A13

Annexin XIII, Annexin, intestine-specific, ISA

ANXA13 (ANX13)

~ 35 kD



Antibodies and Proteins

For studying the function of Annexin, an antibody panel can be a useful tool. We have assorted a vast selection of antibodies and proteins (see table below).

Nearly all listed antibodies are suitable for detection of human annexin in Western blotting. Some Annexin antibodies have already been evaluated for us on formalin-fixed, paraffin embedded sections. The pictures below give samples of such results.

Also included in our Annexin antibody panel ar antibodies that crossreact with other species, like mouse, rat, cattle or pig.



Immunohistochemical  staining of paraffin sections from human intestine using Annexin A4 antibody ARP36686. Arrows indicate Annexin A4 positive epithial  cells of intestinal villus.Immunohistochemical  staining of paraffin sections from human spleen using Annexin A3 antibody ARP36578. Arrows indicate Annexin A3 positive cells.Immunohistochemical  staining of paraffin sections from human intestine using Annexin A1 antibody ARP36568. Arrows indicate Annexin A1 positive epithelial  cells.

Fig.1: Annexin A4 Staining Immunohistochemical staining of paraffin sections from human intestine using Annexin A4 antibody ARP36686. Arrows indicate Annexin A4 positive epithial cells of intestinal villus.

Fig.2: Annexin A3 Staining Immunohistochemical staining of paraffin sections from human spleen using Annexin A3 antibody ARP36578. Arrows indicate Annexin A3 positive cells.

Fig.3: Annexin A1 Staining Immunohistochemical staining of paraffin sections from human intestine using Annexin A1 antibody ARP36568. Arrows indicate Annexin A1 positive epithelial cells.



Reference:

Gerke, V and Moss, SE (2002) Annexins: From Structure to Function. Physiol. Rev. 82 (2): 331-71

Monastyrskaya K, Babiychuk EB, Hostettler A, Rescher U, Draeger A. Annexins as intracellular calcium sensors. Cell Calcium. 2007 Mar;41(3):207-19.

Skrahina T, Piljic A, Schultz C. Heterogeneity and timing of translocation and membrane-mediated assembly of different annexins. Exp Cell Res. 2008 Mar 10;314(5):1039-47.



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Annexin A1 / ANXA1 (N-term) antibody

AP15349PU ANXA1 antibody staining of Human Placenta. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

Annexin A1 / ANXA1 (N-term) antibody

AP15349PU ANXA1 antibody staining of Human Placenta. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

Annexin A1 / ANXA1 (Center) antibody

Western blot analysis of ANKRD6 Antibody (Center) (AP17109PU-N) in A375 cell line lysates (35 µg/lane). ANKRD6 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Annexin A1 / ANXA1 antibody

ELISA: Annexin A1 Antibody [25950002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

Annexin A1 / ANXA1 antibody

Annexin A1 / ANXA1 Mouse IgG1 5E4/1 Purified Hu E, ICC/IF, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

Annexin A1 / ANXA1 antibody

Annexin A1 / ANXA1 Mouse IgG1 6E4/3 Purified Hu E, ICC/IF, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

Annexin A1 / ANXA1 antibody

Western Blot: Annexin I Antibody [NBP1-18842]- (0.5µg/ml) staining of A431 lysate (35µg protein in RIPA buffer) Goat IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Annexin A1 / ANXA1 antibody

Western Blot: Annexin I Antibody [NBP1-19725] - extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Annexin A1 / ANXA1 antibody

Immunohistochemistry:  Annexin A1 Antibody [NBP1-33684] - Immunohistochemical analysis of  Annexin A1 on paraffin-embedded N87 xenograft, using NBP1-33684. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Annexin A2 / ANXA2 antibody

Immunohistochemistry: Annexin A2 Antibody [NBP1-31310] - Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using Annexin A2 antibody at 1:100 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Annexin A2 / ANXA2 (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Annexin A2 / ANXA2 (C-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Annexin A2 / ANXA2 antibody

Mouse; . Lanes: Lane 1: 20ug mouse GL261 cell lysate. Lane 2: 20ug mouse astrocyte cell lysate. Primary Antibody Dilution: 1:250. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:500. Gene Name: ANXA2. Submitted by: Anonymous .; ANXA2 antibody - C-terminal region (ARP36588_T100) in Mouse cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Annexin A2 / ANXA2 antibody

Western Blot: Annexin A2  Antibody [NB100-881] - staining (0.5ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Annexin A2 / ANXA2 antibody

Annexin A2 / ANXA2 Mouse IgG2a 3D5/4 Purified Hu C, E, IP, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Annexin A2 / ANXA2 antibody

Immunohistochemistry: Annexin A2  Antibody [NBP1-19810] -  paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Annexin A3 / ANXA3 (N-term) antibody

Western blot analysis of anti-ANXA3 Antibody (N-term) (Cat.#AP12480PU-N) in Hela cell line lysates (35ug/lane). ANXA3(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Annexin A3 / ANXA3 antibody

Western blot analysis of anti-ANXA3 Antibody (Center) (Cat.#AP12481PU-N) in mouse lung tissue lysates (35ug/lane). ANXA3(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Annexin A3 / ANXA3 antibody

Human Spleen; ANXA3 antibody - N-terminal region (ARP36578_T100) in Human Spleen cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

Annexin A3 / ANXA3 antibody

Human HepG2; WB Suggested Anti-ANXA3 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; ANXA3 antibody - C-terminal region (ARP36579_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

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