Antibodies to Adrenergic Receptors - FocusOn 047

Introduction: Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine.

Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/C, 1B, and 1D), three alpha-2 AR (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3).

ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence.

A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP70), proto-oncogene expression, and mitogenesis have been linked to A1AR activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.

A2AR subtypes inhibit adenylyl cyclase, suppress voltage-sensitive calcium channels, and activate receptor-dependent potassium channels. All of the A2AR subtypes inhibit adenylyl cyclase through coupling to members of the Gi/Go class of G proteins. They are an essential component of the neural circuitry regulating cardiovascular physiology. The physiological function of the A2ARs in the kidney is to regulate sodium/hydrogen exchange although the role of A2AR subtypes remains to be precisely determined.

Beta ARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.

The beta-2 adrenoceptor (B2AR ) is an Adrenergic Receptor expressed in smooth muscle and metabolic tissues. Activation of this receptor induces a decrease in gastrointestinal motility, bronchodilation, vasodilation in skeletal and cardiac muscle, and glycogenolysis in liver. The beta-2 adrenoceptor is also a major lipolytic receptor in human fat cells and may be involved in obesity.

Beta-3 Adrenoceptor (B3AR ) is an Adrenergic Receptor that stimulates lipolysis and increases fatty acids in the blood. Stimulation of the B3AR leads to lipolysis in white adipocytes and nonshivering thermogenesis in brown fat. The beta-3 adrenoceptor has also been suggested to affect the physiological control of cardiac and vascular contractility; beta-3 adrenoceptor stimulation decreases cardiac contractility through activation of a nitric oxide synthase pathway.

Antibody Tools for the Detection of Adrenergic Receptors

Acris Antibodies offers a broad range of antibodies to Adrenergic Receptors, suitable for immunological detection methods like immunohistochemistry, immunofluorescence microscopy, Western blotting and other applications.



Immunohistochemical staining of Adrenal (Medulla) using anti- ADRA1B antibody SP4059P
Immunohistochemical staining of Adrenal (Medulla) using anti- ADRA1B antibody SP4059P Cat.-No. SP4059P
 Immunohistochemical staining of Brain using anti-ADRA1A antibody SP4061P.
Immunohistochemical staining of Brain using anti-ADRA1A antibody SP4061P. Cat.-No. SP4061P
Immunohistochemical staining of Prostate using anti- ADRA1D antibody SP4062P
Immunohistochemical staining of Prostate using anti- ADRA1D antibody SP4062P Cat.-No. SP4062P
AP09613PU-N ADRB1 antibody staining of Human heart lysateat 0.5µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP09613PU-N ADRB1 antibody staining of Human heart lysateat 0.5µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Cat.-No. AP09613PU-N
Immunohistochemistry analysis of Paraffin-Embedded Human brain tissue, using Adrenergic Receptor beta-2 antibody. The picture on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of Paraffin-Embedded Human brain tissue, using Adrenergic Receptor beta-2 antibody. The picture on the right is treated with the synthesized peptide. Cat.-No. AP31835PU-N

 

SP4073P ADRB3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Adipose tissue.
SP4073P ADRB3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Adipose tissue. Cat.-No.

Available antibody panel to Adrenergic Receptor Family

Adrenergic receptor alpha 1 (A1AR), Adrenergic receptor alpha 1b (A1bAR, alpha 1b adrenoceptor), Adrenergic receptor alpha 1c (A1cAR, alpha 1c adrenoceptor), Adrenergic receptor alpha 2a (A2aAR, alpha 2a-adrenoceptor), Adrenergic receptor alpha 2b (A2bAR, alpha 2b-adrenoceptor), Adrenergic receptor alpha 2c (A2cAR, alpha 2c-adrenoceptor), Adrenergic receptor beta 1 (B1AR, beta-1 adrenoceptor), Adrenergic receptor beta 2 (B2AR, beta-2 adrenoceptor), Adrenergic receptor beta 3 (B3AR, beta-3 adrenoceptor) -antibodies



  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (1)

Items 1 to 20 of 22 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-1A adrenergic receptor / ADRA1A (C-term) antibody

Staining of Human Bone Prostate lysate using Alpha-1A adrenergic receptor antibody Cat.-No AP32157PU-N at 1 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Alpha-1A adrenergic receptor / ADRA1A antibody

Immunohistochemistry (IHC) analyzes of AR-alpha-1A antibody (Cat.-No.: AP06787PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-1A adrenergic receptor / ADRA1A antibody

Human Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Alpha-1A adrenergic receptor / ADRA1A (3rd cytopl. dom.) antibody

Figure 1. Immunohistochemical staining of Brain using anti-ADRA1A antibody SP4061P. Rabbit Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Sh ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

Alpha-1B adrenergic receptor / ADRA1B (252-264) antibody

Staining of Human Cerebellum lysate using Alpha-1B adrenergic receptor antibody Cat.-No AP32156PU-N at 0.3 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Alpha-1B adrenergic receptor / ADRA1B antibody

Western blot (WB) analysis of AR-β1 antibody (Cat.-No.: AP06789PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-1B adrenergic receptor / ADRA1B (Center) antibody

Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-2A adrenergic receptor / ADRA2A antibody

Immunohistochemistry (IHC) analyzes of AR alpha2A antibody (Cat.-No.: AP20407PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-2A adrenergic receptor / ADRA2A (C-term) antibody

Rat tissue sections (liver). Immunuhistochemistry on paraffin section, staining ADRA2 in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rb, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Alpha-2B adrenergic receptor / ADRA2B antibody

Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-2B adrenergic receptor / ADRA2B antibody

Figure 1. Western blot analysis using AM05339PU-N ADRA2B antibody on MDCK cells transfected to produce alpha2B receptor protein. Mouse IgG1 5G10 Purified Ms, Rt C, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

Alpha-2C adrenergic receptor / ADRA2C antibody

Western blot (WB) analysis of AR-α2C antibody (Cat.-No.: AP06679PU-N) at 1/500 dilution Lane1:Jurkat whole cell lysate Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate Lane4:Rat kidney tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with BAR2 Antibody  (AP13972PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor antibody

Beta-2 adrenergic receptor Mouse IgG1 6H8 Purified GP, Hu, Rt E, E(detection), FN
0.1 mg / €390.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor antibody

Western blot (WB) analysis of AR-beta 2 antibody (Cat.-No. AP01192PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor pSer261 antibody

Dot blot analysis of anti-Phospho-BAR2-pSer261 Antibody (Cat.#AP12960PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor (N-term) antibody

Western blot analysis of anti-BAR2 Antibody (N-term)(AP13971PU-N) in HL60 cell line lysates (35 µg/lane). BAR2(arrow) was detected using the purified Pab (1:440 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor antibody

Western blot analysis of anti-ADRB2 Antibody (AP13973PU-N) in NCI-H460 cell line lysates (35µg/lane). ADRB2 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor antibody

Western blot analysis of anti-ADRB2 Antibody (AP13974PU-N) in SK-BR-3 cell line lysates (35 µg/lane). ADRB2 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-2 adrenergic receptor antibody

Immunohistochemistry analysis of AR-β2 antibody (AP20290PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Items 1 to 20 of 22 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn