Antibodies to B-cell Linker Protein (BLNK) - FocusOn 079

Introduction

Linker or adapter proteins provide mechanisms by which receptors can amplify and regulate downstream effector proteins. BLNK (also referred to as BASH or SLP-65) is an adaptor molecule that plays a key role in B cell activation initiated by cross-linking the B cell receptor (BCR).

This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of non-receptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is a cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck. BLNK regulates B cell proliferation and differentiation; the recently reported complete loss or drastic reduction of BLNK expression in ~50% of human childhood pre-B acute lymphoblastic leukaemias (ALL) may be one example for its crucial function.

Antibody Panel to B-cell Linker Protein (BLNK)

Acris Antibodies offers a panel of monoclonal and polyclonal antibodies for the detection of BLNK in human, rat or mouse tissues. Antibodies can be used for Immunohistochemistry, Western blotting or other Immunological methods.

Fig. 1: <span>Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining.</span>
Fig. 1: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining. Cat.-No. AM06644SU-N.
Fig. 2: <span>Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.</span>
Fig. 2: Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. AM06644SU-N

Antibody Panel to BLNK

B-cell Linker Protein (BLNK), BASH, SLP-65, antibody.

Literature

Xu S, Huo J, Chew WK, Hikida M, Kurosaki T, Lam KP. Phospholipase Cgamma2 dosage is critical for B cell development in the absence of adaptor protein BLNK. J Immunol. 2006 Apr 15;176(8):4690-8.

Gupta N, Delrow J, Drawid A, Sengupta AM, Fan G, Gélinas C. Repression of B-cell linker (BLNK) and B-cell adaptor for phosphoinositide 3-kinase (BCAP) is important for lymphocyte transformation by rel proteins. Cancer Res. 2008 Feb 1;68(3):808-14.

Chui, C.W., et al. (2002) BLNK: molecular scaffolding through ‘cis’-mediated organization of signaling proteins. EMBO J. 23:6461-6472 (cites the use of this antibody in Western blotting).

Kabak, S., et al. (2002) The direct recruitment of BLNK to immunoglobulin alpha couples the B-cell antigen receptor to distal signaling pathways. Mol. Cell. Biol. 22(8):2524-2535.

Taguchi, T. et al. (2004) Deficiency of BLNK hampers PLC-gamma 2 phosphorylation and Ca2+ influx induced by the pre-B cell receptor in human pre-B cells.Immunology 112(4): 575-582.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

B-cell linker protein / BLNK antibody

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining. Mouse IgG1 5G9 Ascites Hu, Ms E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK (C-term) antibody

TA302649 staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK antibody

Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse BLNK antibody. Rabbit Purified Ms WB
0.1 mg / €260.00
  Acris Antibodies GmbH

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