Antibodies to Catenins - FocusOn 107

Introduction: Catenins are cytoplasmic linker proteins of cell-cell junctions

This protein family include the alpha-, beta-, and gamma-Catenins. In adherens junctions, alpha-Catenin links the Cadherin/beta-Catenin complex to the actin-based cytoskeleton via a direct association between alpha Actinin and alpha Catenin. Beta-Catenin was originally identified as a binding protein of Cadherin. Beta-Catenins associate with the cytoplasmic domain of the adhesion molecules N- and E-Cadherin and are essential for Cadherin function in cell-cell adhesion. It plays a dual role as structural component of cell-cell junctions and as regulatory protein in the nucleus. Beta-Catenin can transduce changes in cell adhesion and junction formation to control transmembrane signaling and gene expression. Delta Catenin is present in adhering type junctions in the nervous system, primarily expressed in the brain and is described to play a role in familial Alzheimer’s disease. Gamma Catenin (Plakoglobin) is found in desmosomes and adherens junctions (see Newsletter Summer 2007 for more detailed information).

Antibody Tools for the Detection of Catenins alpha, beta, gamma and delta

Table 1 shows Acris wide range of antibodies for detection of Catenin family members. Most of these anti-bodies are suitable for Western blots and immunohistochemistry. Please note, that some products do include a positive control for use in Western blots and that we also offer antibodies for detection of phosphorylated beta-Catenin.



Phosphospecificity. Whole cell extracts of control (1) or pervanadate treated (2) SW480 tumor cells were applied to SDS-PAGE and transferred to a PVDF

 

Fig.1: Phosphospecificity. Whole cell extracts of control (1) or pervanadate treated (2) SW480 tumor cells were applied to SDS-PAGE and transferred to a PVDF. The immunoblot was probed with beta-Catenin antibody (phosphoTyr86) cat. no. AM00015PU-N.



Literature:

Conacci-Sorrell, M; Zhurinsky, J; Ben-Ze'ev, A (2002) The cadherin-catenin adhesion system in signaling and cancer. J. Clin. Invest. 109:987-991.

Suzuki SC, Takeichi M. Cadherins in neuronal morphogenesis and function. Dev Growth Differ, 2008 Jun; 50 Suppl 1: 119-130.

Available Antibody Panel to Catenin protein family

All Catenin antibody products: Alpha Catenin antibody, beta Catenin antibody, gamma Catenin (Plakoglobin)) antibody, delta Catenin antibody.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Catenin alpha-1 / CTNNA1 (incl. pos. control) antibody

Detection of endogenous alpha catenin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab a-Cat-15D9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 15D9 Purified Can, Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 30min), using, Alpha-Catenin Antibody (#AP0876PU) and Alpha-Catenin (Phospho-Ser641) Antibody (#AP09467PU). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 30min), using, Alpha-Catenin Antibody (#AP0876PU) and Alpha-Catenin (Phospho-Ser641) Antibody (#AP09467PU). Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 pSer641 antibody

Western blot analysis of extracts from Hela cells untreated or treated with starvation using Alpha-Catenin pSer641 antibody (Cat.-No AP09467PU). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 pSer641 antibody

Western blot analysis of extracts from Hela cells untreated or treated with starvation using Alpha-Catenin pSer641 antibody (Cat.-No AP09467PU). Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

Catenin alpha-1 / CTNNA1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ARRB2 and CTNNA1. HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 and anti-CTNNA1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 4G6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Catenin alpha-1 / CTNNA1 antibody

CTNNA1 polyclonal antibody ( Cat # PAB7024 ) ( 1 µg/ml ) staining of human colon lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Catenin alpha-1 / CTNNA1 antibody

Western blot analysis of extract from A-431 cells, untreated or treated with EGF ( 200ng/ml, 30min ), using CTNNA1 polyclonal antibody ( Cat # PAB12225, Lane 1 and 2 ). Rabbit Hu, Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Catenin alpha-1 / CTNNA1 antibody

Western blot analysis of extract from A-431 cells, untreated ortreated with EGF ( 200ng/ml, 30min ), using CTNNA1 polyclonal antibody (Cat # PAB12225 ) and CTNNA1 (phospho S641) polyclonal antibody ( Cat # PAB14159 ). Rabbit Hu, Ms WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Catenin alpha-1 / CTNNA1 antibody

Immunohistochemistry-Paraffin: Catenin Alpha 1 Antibody [NB100-2917] - (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Catenin alpha-1 / CTNNA1 antibody

Catenin alpha-1 / CTNNA1 Mouse IgG1 1G5 Purified Hu ICC/IF, IP, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Catenin alpha-1 / CTNNA1 antibody

Immunohistochemistry: Catenin-alpha1/2 Antibody [NB100-91684] -  Catenin-alpha1/2 (P940) antibody [NB100-91684] - in paraffin-embedded human lung carcinoma tissue Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Catenin alpha-1 / CTNNA1 antibody

Immunohistochemistry: Catenin Alpha 1 Antibody (EP1793Y) [NB110-55563] - Immunohistochemical staining of paraffin-embedded human breast carcinoma Rabbit IgG EP1793Y Aff - Purified Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Catenin alpha-1 / CTNNA1 antibody

Western Blot: Catenin (alpha) Antibody [NB120-2364] - Western blot analysis of human lung cell extract with catenin-alpha antibody. Rabbit IgG Purified Hu, Ms, Xen C, P, WB
0.5 ml / €440.00
  Novus Biologicals Inc.

Catenin alpha-1 / CTNNA1 antibody

Western Blot: [NB120-11346]-alpha-E-Catenin antibody Rat embryonic brain (S1) extract Rabbit IgG Purified Hu ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Catenin alpha-2 / CTNNA2 antibody

CTNNA2 MaxPab polyclonal antibody. Western Blot analysis of CTNNA2 expression in rat brain. Mouse Serum Hu, Rt E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Catenin alpha-2 / CTNNA2 antibody

Western Blot analysis of CTNNA2 expression in transfected 293T cell line (H00001496-T02) by CTNNA2 MaxPab polyclonal antibody.

Lane 1: CTNNA2 transfected lysate(100.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Catenin alpha-2 / CTNNA2 antibody

Catenin alpha-2 / CTNNA2 Rabbit IgG Purified Hu WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Catenin alpha-3 / CTNNA3 (164-177) antibody

Immunohistochemistry on Paraffin Section of Human heart. Mouse IgG2a 892_24D2S Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Catenin alpha-3 / CTNNA3 antibody

CTNNA3 MaxPab polyclonal antibody. Western Blot analysis of CTNNA3 expression in rat brain. Mouse Serum Hu, Rt E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

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