Antibodies to Cullin Protein Family - FocusOn 057

Cullin-1, 2, and 3 are members of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b, and -5) homologous to the S. cerevisiae cdc53 gene. Cullin-1 forms a complex with human p19skp1 and F box protein p45skp2, both of which, together with cdc53 (known as CUL A), as three subunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The CUL-1/p19skp1/p45skp2 complex is thought to play a role in the ubiquitination of G1 regulatory proteins. Instead, Cullin-2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown. Cullin-3 is proposed to be a part of E3 proteinubiquitin ligase. It forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by protesomes. Cyclin E is an activator of cdk2, which leads the cell to S phase.

Cul-3-/- mice show increased accumulation of Cyclin E protein and disruption of normal cell cycle regulation in vivo. Thus Cullin-3 is important in the regulation of S phase of cell cycle.

Cullins 4a, 5 and 7 are also essential components of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.

Antibody Tools for the Detection of Cullins

Acris Antibodies offers a panel of antibodies to various Cullins. These antibodies can be used for immunological detection of human and mouse Cullin proteins by Western blot, immunohistochemistry, ELISA or immunoprecipitation.

Staining of formalin fixed paraffin embedded sections of human tonsils using Cullin-1 antibody (AP00497PU-N) (Fig.1), Cullin-2 antibody (AP00499PU-N) (Fig.2) and Cullin-3 antibody (AP00558PU-N) (Fig.3). Note nuclear staining of epithelial cells.

Available Antibody Panel to Cullins

Anti- Cullin-1 (CUL-1, CUL1), Cullin-2 (CUL-2, CUL2), Cullin-3 (CUL-3, CUL3), Cullin-4A (CUL-4A, CUL4A), Cullin-5 (CUL-5, CUL5, VACM-1), and Cullin-7 (CUL-7, CUL7, KIAA0076) antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cullin 1 antibody

Human Jurkat; WB Suggested Anti-CUL1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; CUL1 antibody - C-terminal region (AVARP03049_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

Cullin-3 antibody

Western Blot: Cul3 Antibody [NB100-58787] - Detection of Human Cul3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-Cul3 antibody NB100-58787 used for IP at 3 mcg/mg lysate. Cul3 was also immunoprecipitated by other rabbit anti-Cul3 antibodies NB100-58788 and NB100-58789, which recognize downstream epitopes.  Detection:  Chemiluminescence with an exposure time of 10 seconds. Rabbit Aff - Purified Hu ICC/IF, IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

Cullin-3 antibody

Western Blot: Cul3 Antibody [NB100-58788] -  Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes). Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Cullin-5 antibody

Human HepG2; WB Suggested Anti-CUL5 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; CUL5 antibody - C-terminal region (ARP35127_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Dros, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

Cullin-7 antibody

Immunoprecipitation: Cul-7 Antibody [NB100-602] - Detection of Human and Mouse Cul7 by Immunoprecipitation using NB100-602. Samples: Whole cell lysate (1 mg) from human U2OS (H) and mouse wild-type MEF (M) cells.  Western blot of immunoprecipitates was performed using another rabbit anti-Cul7 antibody. Detection: Chemiluminescence with an exposure time of 15 seconds. Rabbit Aff - Purified Hu, Ms IP
0.1 ml / €440.00
  Novus Biologicals Inc.

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