Antibodies to Cullin Protein Family - FocusOn 057

Cullin-1, 2, and 3 are members of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b, and -5) homologous to the S. cerevisiae cdc53 gene. Cullin-1 forms a complex with human p19skp1 and F box protein p45skp2, both of which, together with cdc53 (known as CUL A), as three subunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The CUL-1/p19skp1/p45skp2 complex is thought to play a role in the ubiquitination of G1 regulatory proteins. Instead, Cullin-2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown. Cullin-3 is proposed to be a part of E3 proteinubiquitin ligase. It forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by protesomes. Cyclin E is an activator of cdk2, which leads the cell to S phase.

Cul-3-/- mice show increased accumulation of Cyclin E protein and disruption of normal cell cycle regulation in vivo. Thus Cullin-3 is important in the regulation of S phase of cell cycle.

Cullins 4a, 5 and 7 are also essential components of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.

Antibody Tools for the Detection of Cullins

OriGene Technologies offers a panel of antibodies to various Cullins. These antibodies can be used for immunological detection of human and mouse Cullin proteins by Western blot, immunohistochemistry, ELISA or immunoprecipitation.

Staining of formalin fixed paraffin embedded sections of human tonsils using Cullin-1 antibody (AP00497PU-N) (Fig.1), Cullin-2 antibody (AP00499PU-N) (Fig.2) and Cullin-3 antibody (AP00558PU-N) (Fig.3). Note nuclear staining of epithelial cells.

Available Antibody Panel to Cullins

Anti- Cullin-1 (CUL-1, CUL1), Cullin-2 (CUL-2, CUL2), Cullin-3 (CUL-3, CUL3), Cullin-4A (CUL-4A, CUL4A), Cullin-5 (CUL-5, CUL5, VACM-1), and Cullin-7 (CUL-7, CUL7, KIAA0076) antibody

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