Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD) - FocusOn 092

Introduction

The DNA fragmentation factor (DFF) is composed of two subunits, the 40-kDa caspase-3-activated nuclease (DFF40/CAD) and its 45-kDa inhibitor (DFF45/ICAD).

Apoptosis is induced through a number of cell death receptors and their ligands like TNF and TNF receptor. The signaling cascade is carried further by adaptor proteins containing death domains and by caspase family of proteins. The caspase-activated DNase (CAD) is the primary nuclease responsible for oligonucleosomal DNA fragmentation during apoptosis. DFF45/ICAD acts as a chaperon for appropriate folding of DFF40 and it also acts as inhibitor of DFF40. Caspase 3 upon activation cleaves DFF to release DFF40/CAD, which oligomerizes into a large active complex and causes the degradation of DNA in nuclei. Hence cleavage of DFF45 is an important step in apoptosis.

Antibody Panel to DFF (CAD and ICAD)

Acris Antibodies offers a range of monoclonal and polyclonal antibodies to DFF40 and DFF45 suitable for Western blotting, Immunohistochemistry and / or other immunological detection methods.

Fig. 1: <br>Anti-DFF40 (CAD) Western blot analysis of 30 µ g of HeLa cell lysate with antibody SM7025P at a dilution of 2 µ g/ml. A 40 kDa band is observed.Fig. 2: <br>Anti-DFF45 (ICAD) Western blot analysis of 50 µ g of total cell lysate from HeLa cells with antibody SP7059P at 1:2000 dilution. A 4 5 kDa band is observed.Fig. 3: <br>Anti-DFF45 (ICAD) Western blot of antibody SP5430P in HeLa cell lysateFig. 4: <br>Anti-DFF45 (ICAD) Western blot of antibody SP5431P in HeLa cell lysate

Fig. 1: Anti-DFF40 (CAD) Western blot analysis of 30 µg of HeLa cell lysate with antibody SM7025P at a dilution of 2 µg/ml. A 40 kDa band is observed.

Fig. 2: Anti-DFF45 (ICAD) Western blot analysis of 50 µg of total cell lysate from HeLa cells with antibody SP7059P at 1:2000 dilution. A 45 kDa band is observed.

Fig. 3: Anti-DFF45 (ICAD) Western blot of antibody SP5430P in HeLa cell lysate.

Fig. 4: Anti-DFF45 (ICAD) Western blot of antibody SP5431P in HeLa cell lysate.

Antibody Panel to DFF

DNA Fragmentation Factor DFF40 (CAD) and DFF45 (ICAD) antibody.

Literature

Neimanis S, Albig W, Doenecke D, Kahle JSequence elements in both subunits of the DNA fragmentation factor are essential for its nuclear transport.J Biol Chem. 2007 Dec 7;282(49):35821-30. Epub 2007 Oct 15.

Lechardeu D, Dougaparsad S, Nemes C, Lukas GL.Oligomerization state of the DNA fragmentation factor (DFF) in normal and apoptotic cells.J Biol Chem. 2005 Dec 2; 280(48): 40216-25.

Widlak P, Garrard WT.Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD andendonuclease G. J Cell Biochem. 2005 Apr 15; 94(6): 1078-87

Korn C, Scholz SR, Gimadutdinow O, Lurz R, Pingoud A, Meiss G.Interaction of DNA Fragmentation Factor (DFF) with DNA reveals an unprecedentedmechanism for Nuclease inhibition and suggests that DFF can be activated in a DNA-boundstate. J Biol Chem. 2005 Feb 18; 280 (7): 6005-6015

Eckhart L, Fischer H, Tschachler E.Phylogenomics of caspase-activated DNA fragmentation factor.Biochem Biophys Res Commun. 2007 Apr 27;356(1):293-9.

Inohara N, Koseki T, Chen S, Benedict MA, Núñez G. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD.J Biol Chem. 1999 Jan 1;274(1):270-4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DFFA / ICAD antibody

DFFA MaxPab rabbit polyclonal antibody. Western Blot analysis of DFFA expression in human pancreas. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Colon carcinoma stained with Anti-DFF-45 antibody Rabbit Purified Hu P, WB
1 ml / €440.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

Colon carcinoma stained with Anti-DFF-45 antibody Rabbit Purified Hu P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

Immunohistochemistry (IHC) analyzes of ICAD antibody (Cat.-No.: AP06798PU-N) in paraffin-embedded human breast carcinoma tissue Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

Figure 1.: Immunohistochemical staining of  Formalin-Fixed Paraffin-Embedded (FFPE) prostate tissue using antibody AP07742PU-N Rabbit Purified Hu ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DFFA / ICAD (N-term) antibody

Immunohistochemical staining of mouse lung with Rabbit anti Mouse ICAD antibody Cat.-No SP1232P Rabbit IgG Purified Ms P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DFFA / ICAD (C-term) antibody

Western blot analysis of lysates from mouse lung (L), brain (B), liver (L) and kidney (K) probed with Rabbit anti Mouse ICAD) (SP1235P) Rabbit Purified Ms WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

DFFA MaxPab rabbit polyclonal antibody. Western Blot analysis of DFFA expression in human pancreas. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and DFFA. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-DFFA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 3A11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Immunoblot analysis of cell lysates.
Lane 1 : HeLa cell lysate.
 Lnae 2 : A-431 cell lysate.
Lane 3 : HepG2 cell lysate.
Lane 4 : 293T cell lysate.
Lane 5 : Jurkat cell lysate. Mouse AF24G5 Hu, Ms, Rt E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Immunoprecipitation analysis of Jurkat lysates.
Lane 1 : Input.
Lane 2 : Precipitatates.
Western Blotting by DFFA monoclonal antibody, clone AF41E3 ( Cat # MAB2703 ). Mouse AF41E3 Hu, Ms, Rt E, IP
0.1 ml / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Immunoblot analysis of cell lysates. Lane 1 : HeLa cell lysate. Lane 2 : Jurkat cell lysate. Lane 3 : 293T cell lysate. Lane 4 : A-431 cell lysate. N.S: Non specific band. Rabbit Hu WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

DFFA / ICAD Rabbit Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Immunohistochemical staining of DFFA on formalin fixed, paraffin embedded colon cancer with DFFA polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Immunocytochemistry of DFFA in HeLa cells with DFFA polyclonal antibody ( Cat # PAB13068 ) at 5 µg/ml. Rabbit Hu ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Western blot analysis of DFFA in HeLa, ( H ), K-562 ( K ), Jurkat ( J ), and Raji ( R ) whole cell lysate with DFFA polyclonal antibody ( Cat # PAB13069 ) at 1 : 1000 dilution. Rabbit Hu WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Immunohistochemistry of Dffa in mouse lung tissue with Dffa polyclonal antibody ( Cat # PAB13071 ) at 2 µg/ml. Rabbit Ms P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Western blot analysis of Dffa in mouse lung ( L ), brain ( B ), liver ( L ), and kidney ( K ) tissue lysate with Dffa polyclonal antibody ( Cat # PAB13072 ) at 1 : 1000 dilution. Rabbit Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Western blot analysis of 50 µg of murine kidney tissue lysate with Dffa polyclonal antibody ( Cat # PAB13556 ) at 1 : 500 dilution. Rabbit Ms WB
0.1 mg / €820.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Western blot analysis of 25 µg of tissue lysatefrom murine lung ( Lane 1 ) and spleen ( Lane 2 ) , respectively, with Dffa polyclonal antibody ( Cat # PAB13557 ) at 1 : 500 dilution. Rabbit Ms WB
0.1 mg / €820.00
  Abnova Taiwan Corp.

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