Antibodies to Dynamins - FocusOn 091

Introduction

Membrane transport between compartments in eukaryotic cells requires proteins that allow the budding and scission of nascent cargo vesicles from one compartment and their targeting and fusion with another. Dynamins are involved in the scission of a wide range of vesicles and organelles as well as in many processes including budding of transport vesicles, division of organelles, cytokinesis and pathogen resistance.

The Dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between Dynamin and other cellular proteins.

There are also at least three isoforms of this protein. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. The dynamins participate in the cellular process of clathrin-mediated and fluidphase endocytosis.

Antibody Panel to Dynamins

Acris Antibodies offers a range of monoclonal and polyclonal antibodies to Dynamins isoforms 1-3 suitable for Western blotting, Immunohistochemistry/ Immunofluorescence.and / or other immunological detection methods.

Western blot of Dynamin 1 on rat brain homogenate using antibody Cat.No. SP5239P.Western blot of Dynamin 2 on mouse testes homogenate using antibody Cat.No. SP5240P.Western blot using antibody Cat.No. NB300-209 specific for Dynamin phosphorylated at Ser 774. A band representing Dynamin is seen at ~ 97 kD. The labeling of the Dynamin bands in Western Blots of rat brain is blocked by the phosphopeptide.

Fig. 1: Western blot of Dynamin 1 on rat brain homogenate using antibody Cat.No. SP5239P.

Fig. 2: Western blot of Dynamin 2 on mouse testes homogenate using antibody Cat.No. SP5240P.

Fig. 3: Western blot using antibody Cat.No. NB300-209 specific for Dynamin phosphorylated at Ser 774. A band representing Dynamin is seen at ~ 97 kD. The labeling of the Dynamin bands in Western Blots of rat brain is blocked by the phosphopeptide.

Antibody Panel to Dynamins

Dynamins (DNM1, DNM2 and DNM3) antibody.

Literature

Wells RC, Picton LK, Williams SC, Tan FJ, Hill RB.Direct binding of the dynamin-like GTPase, Dnm1, to mitochondrial dynamics protein Fis1 is negatively regulated by the Fis1 N-terminal arm.J Biol Chem. 2007 Nov 16;282(46):33769-75. Epub 2007 Sep 20

Naylor K, Ingerman E, Okreglak V, Marino M, Hinshaw JE, Nunnari J.Mdv1 interacts with assembled dnm1 to promote mitochondrial division.J Biol Chem. 2006 Jan 27;281(4):2177-83. Epub 2005 Nov 4.

Ingerman E, Perkins EM, Marino M, Mears JA, McCaffery JM, Hinshaw JE, Nunnari J.Dnm1 forms spirals that are structurally tailored to fit mitochondria.J Cell Biol. 2005 Sep 26;170(7):1021-7.

Reubold TF, Eschenburg S, Becker A, Leonard M, Schmid SL, Vallee RB, Kull FJ, Manstein DJ.Crystal structure of the GTPase domain of rat dynamin 1.Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13093-8.

Carey RM, Balcz BA, Lopez-Coviella I, Slack BE.Inhibition of dynamin-dependent endocytosis increases shedding of the amyloid precursorprotein ectodomain and reduces generation of amyloid beta protein.BMC Cell Biol. 2005 Aug 11;6:30.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Dynamin-1 pSer774 antibody

Figure 1 Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser774 Dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Sheep IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Dynamin-1 pSer774 antibody

Dynamin-1 Sheep IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt C, ICC/IF, WB
0.1 ml / €500.00
  Acris Antibodies GmbH

Dynamin-1 pSer778 antibody

Dynamin-1 Sheep IgG Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

Dynamin-1 antibody

Immunohistochemistry analyzes of Dynamin-1 antibody (Cat.-No.: AP06385PU-N) in Paraffin-Embedded Human tbrain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Dynamin-1 (C-term) antibody

AP08309PU-N Dynamin-1 antibody staining of Formalin-Fixed Paraffin Embedded Human Brain, Cortex at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Dynamin-1 pSer778 antibody

Figure 1 Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 Dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Sheep IgG Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Dynamin-1 (1-750) antibody

Dynamin-1 Mouse IgG D5 Purified Bov, Chk, Hu, Ms, Rt F, IP, P, WB
50 µg / €340.00
  Novus Biologicals Inc.

Dynamin-1 (N-term) antibody

Western Blot: Dynamin Antibody [NB100-61094] - Rat cortex lysate showing labeling of the ~95k Dynamin protein. Rabbit IgG Aff - Purified Rt WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Dynamin-1 antibody

Dynamin-1 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Dynamin-1 antibody

Immunofluorescent: Dynamin-1 Antibody (EP772Y) [NB110-56933] - Immunofluorescent staining of PC12 cells using anti-Dynamin-1.jpg (cat. #110-56933). Rabbit IgG Aff - Purified Hu ICC/IF, IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Dynamin-1 antibody

Immunohistochemistry: Dynamin-1 (C-term) Antibody (EP801Y) [NB110-56934] - Immunohistochemical staining of paraffin-embedded human brain tissue using anti-Dynamin-1 (cat. #110-56934). Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Dynamin-1 pSer774 antibody

Western Blot: Dynamin [phospho Ser774] Antibody [NB300-209] - Dynamin Phosphospecific Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Sheep Aff - Purified Rt ICC/IF, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Dynamin-1 pSer778 antibody

Western blot: Dynamin [phospho Ser778] Antibody [NB300-210] - Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in ?-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment. Sheep Aff - Purified Rt WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Dynamin-2 antibody

Western Blot: Dynamin 2 - Synapse Antibody [NB300-617] - Figure 1 shows a Western blot of Dyn2 on mouse testes homogenate using NB300-617. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Dynamin-3 antibody

AP17991PU-N Dynamin-3 antibody staining of Formalin-Fixed, Paraffin-Embedded Human brain using Peroxidase-conjugated to the secondary antibody, followed by DAB staining. Clinical relevance has not been evaluated. Rabbit Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Dynamin-3 antibody

Western Blot: Dynamin 3 Antibody [NB120-3458] - Western blot of Dyn3 on rat liver extract using NB120-3458. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 ml / €400.00
  Novus Biologicals Inc.

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