Antibodies to Endothelial Differentiation Gene Receptors (EDG-1,-2,-4,-5,-6,-7 and -8) - FocusOn 093

Introduction

Lysophosphatidic acid (LPA), sphingosine 1-phosphate (S1P) and some other structurally related lysophospholipids are active growth factors and stimuli for diverse cellular functions.

LPA and S1P promote early T cell migration to tissue sites of immune responses and regulate T cell proliferation and secretion of numerous cytokines. The endothelial differentiation gene (EDG) receptors are a class of G protein-coupled receptors. EDG-1, -3, -5, -6, and -8 (known as the endothelial differentiaton gene-1 family) regulate diverse processes including cell migration, angiogenesis, vascular maturation, heart development, neurite retraction and soma rounding. These receptors bind the bioactive lipid sphingosine-1-phosphate (S1P) as the primary signaling ligand. EDG-2, -4, and -7 bind the ligand lysophosphatidic acid.

Antibody Panel to EDG Receptors

OriGene Technologies offers a wide range of polyclonal antibodies to EDG-receptors suitable for Immunohistochemistry (on Paraffin-embedded tissues) and Western blotting.

Fig.1: Immunohistochemical staining of heart vessel using antibody Cat.No. SP4617P (anti-EDG-1)

Fig.2: Immunohistochemical staining of brain neurons and glia using antibody SP4371P (anti-EDG-2)

Fig.3: Immunohistochemical staining of breast carcinoma using antibody Cat.No. SP4498P (anti-EDG-5)

Fig.4:Immunohistochemical staining of lymph node using antibody Cat.No. SP4500 (anti-EDG-6)

Fig.5: Immunohistochemical staining of amygdala using antibody Cat.No. SP4375P (anti-EDG-7)

Fig.6: Immunohistochemical staining of neurons and glia in rat brain using antibody Cat.No SP4501 (anti-EDG-8)

Antibody Panel to

Endothelial Differentiation Gene Receptors (EDG-1,-2,-4,-5,-6,-7 and -8) antibody.

Literature

Kohno T, Igarashi Y.Roles for N-glycosylation in the dynamics of EDG-1/S1P1 in sphingosine 1-phosphate stimulated cells.Glycoconj J. 2004; 21(8-9): 497-501.

Laychock SG, Tian Y, Sessanna SM.Endothelial differentiation gene receptors in pancreatic islets and INS-1 cells.Diabetes. 2003 Aug; 52(8): 1986-93.

Huang MC, Graeler M, Shankar G, Spencer J, Goetzl EJ.Lysophospholipid mediators of immunity and neoplasia.Biochim Biophys Acta. 2002 May 23; 1582(1-3): 161-7.

Toman RE, Milstien S, Spiegel S.Sphingosine-1-phosphate: an emerging therapeutic target.Expert Opin Ther Targets. 2001 Feb; 5(1): 109-23.