Antibodies to Endothelial Differentiation Gene Receptors (EDG-1,-2,-4,-5,-6,-7 and -8) - FocusOn 093

Introduction

Lysophosphatidic acid (LPA), sphingosine 1-phosphate (S1P) and some other structurally related lysophospholipids are active growth factors and stimuli for diverse cellular functions.

LPA and S1P promote early T cell migration to tissue sites of immune responses and regulate T cell proliferation and secretion of numerous cytokines. The endothelial differentiation gene (EDG) receptors are a class of G protein-coupled receptors. EDG-1, -3, -5, -6, and -8 (known as the endothelial differentiaton gene-1 family) regulate diverse processes including cell migration, angiogenesis, vascular maturation, heart development, neurite retraction and soma rounding. These receptors bind the bioactive lipid sphingosine-1-phosphate (S1P) as the primary signaling ligand. EDG-2, -4, and -7 bind the ligand lysophosphatidic acid.

Antibody Panel to EDG Receptors

Acris Antibodies offers a wide range of polyclonal antibodies to EDG-receptors suitable for Immunohistochemistry (on Paraffin-embedded tissues) and Western blotting.

Immunohistochemical staining of heart vessel using antibody Cat.No. SP4617P (anti-EDG-1)Immunohistochemical staining of brain neurons and glia using antibody SP4371P (anti-EDG-2)

Fig.1: Immunohistochemical staining of heart vessel using antibody Cat.No. SP4617P (anti-EDG-1)

Fig.2: Immunohistochemical staining of brain neurons and glia using antibody SP4371P (anti-EDG-2)

Immunohistochemical staining of breast carcinoma using antibody Cat.No. SP4498P (anti-EDG-5)Immunohistochemical staining of lymph node using antibody Cat.No. SP4500 (anti-EDG-6)

Fig.3: Immunohistochemical staining of breast carcinoma using antibody Cat.No. SP4498P (anti-EDG-5)

Fig.4:Immunohistochemical staining of lymph node using antibody Cat.No. SP4500 (anti-EDG-6)

Immunohistochemical staining of amygdala using antibody Cat.No. SP4375P (anti-EDG-7)Immunohistochemical staining of neurons and glia in rat brain using antibody Cat.No SP4501 (anti-EDG-8)

Fig.5: Immunohistochemical staining of amygdala using antibody Cat.No. SP4375P (anti-EDG-7)

Fig.6: Immunohistochemical staining of neurons and glia in rat brain using antibody Cat.No SP4501 (anti-EDG-8)

Antibody Panel to

Endothelial Differentiation Gene Receptors (EDG-1,-2,-4,-5,-6,-7 and -8) antibody.

Literature

Kohno T, Igarashi Y.Roles for N-glycosylation in the dynamics of EDG-1/S1P1 in sphingosine 1-phosphate stimulated cells.Glycoconj J. 2004; 21(8-9): 497-501.

Laychock SG, Tian Y, Sessanna SM.Endothelial differentiation gene receptors in pancreatic islets and INS-1 cells.Diabetes. 2003 Aug; 52(8): 1986-93.

Huang MC, Graeler M, Shankar G, Spencer J, Goetzl EJ.Lysophospholipid mediators of immunity and neoplasia.Biochim Biophys Acta. 2002 May 23; 1582(1-3): 161-7.

Toman RE, Milstien S, Spiegel S.Sphingosine-1-phosphate: an emerging therapeutic target.Expert Opin Ther Targets. 2001 Feb; 5(1): 109-23.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EDG1 / S1P1 antibody

Western blot analysis of lysates of RH7777 cells transfected with full lenth human EDG-1 protein using anti-EDG-1 CT antibody at 10 µg/ml (1), antibody preincubated with specific blocking peptide (2) and antibody preincubated with non-specific control peptide (3) using Pierce Femto Signal substrate. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €570.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Western blot (WB) analysis of EDG-1 antibody (Cat.-No.: AP01197PU-N) in extracts from COLO205 cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Western blot (WB) analysis of EDG-1 antibody (Cat.-No.: AP01462PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG1 / S1P1 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

EDG1 MaxPab rabbit polyclonal antibody. Western Blot analysis of EDG1 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Western Blot analysis of EDG1 expression in transfected 293T cell line by EDG1 monoclonal antibody (M03), clone 1F11.

Lane 1: EDG1 transfected lysate(42.8 KDa).
Lane 2: Non-transfected lysate. Mouse IgG3 1F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with S1PR1 polyclonal antibody ( Cat # PAB4718 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Western blot analysis of lysates of McA-RH7777 cells transfected with full length human S1PR1 protein using S1PR1 polyclonal antibody ( Cat # PAB10360 ) at 10 µg/ml (lane 1), antibody preincubated with specific blocking peptide (lane 2) and antibody preincubated with non-specific control peptide (lane 3). Rabbit Hu, Ms, Rt WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Immunohistochemistry: EDG-1 Antibody (MM0044-7M15) [NB110-93513] - Immunohistochemistry-Paraffin: EDG-1 Antibody (MM0044-7M15) [NB110-93513] - Normal Human Placenta  Tissue Mouse IgG1 MM0044-7M15 Purified Hu F, P, WB
0.1 mg / €450.00
  Novus Biologicals Inc.

EDG1 / S1P1 antibody

Immunofluorescent: EDG1 Antibody [NB120-11424] - Figure 1 shows immunofluorescence of S1P1 in transfected CCL39 cells using NB120-11424. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

EDG1 / S1P1 antibody

Western Blot: EDG-1 Antibody [NBP1-00786] - Western blot (WB) analysis of EDG-1 (S232)   antibody in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

EDG1 / S1P1 antibody

Sphingolipid Receptor Edg1/S1P1 detection using NLS 1013 on human brain (Neurons and Glia). Rabbit Aff - Purified Hu P
50 µl / €440.00
  Novus Biologicals Inc.

EDG2 / LPAR1 antibody

Immunohistochemistry (IHC) analysis of EDG-2 antibody (Cat.-No.: AP01253PU-N) in paraffin-embedded mouse brain tissue. Rabbit Aff - Purified Hu, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG2 / LPAR1 (N-term) antibody

The anti-EDG2 N-term Antibody is used in Western blot to detect EDG2 in A375 lysate. Rabbit Ig Purified Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

EDG2 / LPAR1 antibody

EDG2 / LPAR1 Mouse IgG2a 2E2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

EDG2 / LPAR1 antibody

The LPAR1 polyclonal antibody ( Cat # PAB4719 ) is used in Western blot to detect LPAR1 in A-375 cell lysate. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

EDG2 / LPAR1 antibody

Western blot analysis using LPAR1 polyclonal antibody ( Cat # PAB10126 ) on McA-RH7777 cells transfected with LPAR1 protein at 5 µg/ml. Rabbit Hu WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

EDG2 / LPAR1 antibody

Immunohistochemistry: EDG-2 Antibody [NBP1-00788] - Immunohistochemistry (IHC) analyzes of EDG-2 (W45) antibody in paraffin-embedded mouse brain tissue. Rabbit Aff - Purified Hu, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

EDG2 / LPAR1 antibody

EDG2 / LPAR1 Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Sh C, ICC/IF, P, WB
25 µl / €140.00
  Novus Biologicals Inc.

EDG2 / LPAR1 antibody

Adrenal Rabbit Aff - Purified Hu P
50 µl / €440.00
  Novus Biologicals Inc.

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