Antibodies to FLIP/I-FLICE/Casper - FocusOn 103

Introduction: Fas-associated death domain (FADD) and caspase-8 are key signal transducers for death receptor-induced apoptosis; cellular Fas-associated death domain-like interleukin-1-beta converting enzyme (FLICE) inhibitory proteins (cFLIPs) are endogenous caspase homologues that inhibit programmed cell death. Interestingly, FADD and caspase-8 also play a role in T cell development and T cell receptor (TCR)-mediated proliferative responses.

Animal models have indicated that c-FLIP plays an important role in T cell proliferation and heart development. To date, three isoforms of human cytosolic FLIP (c-FLIP) and six viral homologs (v-FLIP) have been identified. Recently, the crystal structure of v-FLIP MC159 was determined for the first time as an atomic-detail FLIP structure, which revealed that two death effector domains are packed tightly against each other mainly through conserved hydrophobic interactions. Abnormal c-FLIP expression has been identified in various diseases such as multiple sclerosis (MS), Alzheimer's disease (AD), diabetes mellitus, rheumatoid arthritis (RA) and various cancers.

Antibody Panel to FLIP/I-FLICE

Acris Antibodies offers a range of polyclonal antibodies to FLICE inhibitory proteins useful for various applications.

Immunocytochemistry: AP09143PU-N FLIP antibody staining of Jurkat cells at 10 µg/ml.

Fig. 1

Western blot: AP09143PU-N FLIP antibody staining of HeLa (H), Jurkat (J) and K562 (K) whole cell lysates at 0.5 µg/ml.

Fig. 2

Fig. 1: Immunocytochemistry: AP09143PU-N FLIP antibody staining of Jurkat cells at 10 µg/ml.

Fig. 2: Western blot: AP09143PU-N FLIP antibody staining of HeLa (H), Jurkat (J) and K562 (K) whole cell lysates at 0.5 µg/ml.

Available Antibody Panel FLIP/I-FLICE/Casper

FLIP/I-FLICE, FLAME-1, CASH, CLARP –antibody.

Literature

Chau H, Wong V, Chen NJ, Huang HL, Lin WJ, Mirtsos C, Elford AR, Bonnard M, Wakeham A,You-Ten AI, Lemmers B, Salmena L, Pellegrini M, Hakem R, Mak TW, Ohashi P, Yeh WC.Cellular FLICE-inhibitory protein is required for T cell survival and cycling.J Exp Med. 2005 Aug 1; 202(3): 405-13.

Hainsworth AH, Bermpohl D, Webb TE, Darwish R, Fiskum G, Qiu J, McCarthy D, Moskowitz MA,Whalen MJ.Expression of cellular FLICE inhibitory proteins (cFLIP) in normal and traumatic murine and humancerebral cortex. J Cereb Blood Flow Metab. 2005 Aug; 25(8): 1030-40.

Micheau O.Cellular FLICE-inhibitory protein: an attractive therapeutic targetExpert Opin Ther Targets. 2003 Aug; 7(4): 559-73.

Hu S, Vincenz C, Ni J, Gentz R, Dixit VM.I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1 and CD95-induced apoptosis.J Biol Chem. 1997 Jul 11; 272(28): 17255-7.

5. Yang JK.FLIP as an Anti-Cancer Therapeutic Target.Yonsei Med J. 2008 Feb;49(1):19-27.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CFLAR / Casper / I-FLICE (1-376) antibody

Western blot analysis: Cell lysates of mouse kidney, MCF7 and rat spleen (40 µg) were resolved by SDS-PAGE, trans-ferred to NC membrane and probed with FLIP antibody Cat.-No. AM09055PU-N  (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 5D8 Purified Hu, Ms, Rt E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE (Long Form) antibody

Western blot analysis of FLIP in HeLa (left), Jurkat (middle) and K562 (right) cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE (206-222) antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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