Antibodies to Flotillin 1 & 2 - FocusOn 102

Introduction

Flotillins are integral membrane proteins that have been shown to be present in several subcellular components, including caveolae (invaginated plasma membrane microdomains), lipid rafts (sphingolipid and cholesterol-rich, detergent-resistant plasma membrane microdomains), and the Golgi apparatus. The molecular function of flotillins remains uncertain. They are probably involved in organising the structure of caveolae and lipid rafts, and other detergent resistant membrane domains. They may also be involved in signal transduction. Flotillins have been shown to accumulate in brain cells with the development of Alzheimer's pathology . Also included in this family are Reggie proteins, which are expressed in non-caveolar neuronal plasma membrane domains.

Antibody Panel to Flotillin

Acris Antibodies offers two polyclonal antibodies to Flotillin 1 and Flotillin 2, respectively. Each Flotillin-antibody is suitable for use in Western blotting and ELISA application (see pictures and table below.)

<b>Flotillin 1:</b> <br>Antibody AP16027PU-N (0.3 µg/ml) staining (0.3 µg/ml) of H460 lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Flotillin 1:
Antibody AP16027PU-N (0.3 µg/ml) staining (0.3 µg/ml) of H460 lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

<b>Flotillin 2:</b> <br>Antibody AP16026PU-N (0.1 µg/ml) staining of human brain (cerebellum) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by western blot using chemiluminescence

Flotillin 2:
Antibody AP16026PU-N (0.1 µg/ml) staining of human brain (cerebellum) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by western blot using chemiluminescence

Immunohistochemical analysis of Paraffin-Embedded Lung CA, using Flotillin-2 antibody (AP20043PU-N at 1/100 dilution).
Immunohistochemical analysis of Paraffin-Embedded Lung CA, using Flotillin-2 antibody (AP20043PU-N at 1/100 dilution).
 

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