Antibodies to Frizzled proteins 1-10 - FocusOn 099

Antibody Tools for the Detection of Frizzled 1-10

Acris Antibodies offers a range of antibodies to frizzled 1-10. These antibodies have been raised against different epitopes but most of them are specific for the N-terminal extracellular domain of the respective proteins. Many of the products have been shown to be suitable for immunohistochemistry on paraffin-embedded tissues.

Introduction

Frizzled proteins (Frizzled, FZD) are 7-transmembrane receptors which act as receptors for Wnt proteins.

Wnt proteins form a family of highly conserved secreted signalling molecules that regulate cell to cell interactions during embryogenesis. The N-terminal cysteine rich domain (CRD) region of frizzled receptors is involved in the ligand-receptor interaction. The binding of a Wnt ligand to frizzled (done with the aid of cofactors) activates frizzled. This, in turn, activates a cytosolic protein called dishevelled (Dsh). Activated Dsh inhibits the beta-catenin degradation complex, so beta-catenin escapes by proteasomes and enters the nucleus where it binds to the promoters and / or enhancers of its target genes.

At present, at least ten different human frizzled genes have been described, playing essential roles in the regulation of embryonic development, tissue and cell polarity, formation of neural synapses and the regulation of proliferation. The signalling pathway has been shown to be implicated in the genesis of numerous human cancers.

Fig. 1: Staining of formalin-fixed, paraffin-embedded (FFPE) human prostate using frizzled-1 antibody Cat.-No. SP4142P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 1: Staining of formalin-fixed, paraffin-embedded (FFPE) human prostate using frizzled-1 antibody Cat.-No. SP4142P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 2: Staining of human brain (neurons and glia, FFPE) using frizzled-3 antibody Cat.-No. SP4568P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 2: Staining of human brain (neurons and glia, FFPE) using frizzled-3 antibody Cat.-No. SP4568P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 3: Staining of human heart (cardiac myocytes, FFPE) using frizzled-7 antibody Cat.-No. SP4150P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 3: Staining of human heart (cardiac myocytes, FFPE) using frizzled-7 antibody Cat.-No. SP4150P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 4: Staining of human pancreas (islet of Langerhans, FFPE) using frizzled-8 antibody Cat.-No. SP4151P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 4: Staining of human pancreas (islet of Langerhans, FFPE) using frizzled-8 antibody Cat.-No. SP4151P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 5: Staining of human brain (Purkinje neurons, FFPE) using frizzled-9 antibody Cat.-No. SP4154P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 5: Staining of human brain (Purkinje neurons, FFPE) using frizzled-9 antibody Cat.-No. SP4154P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 6: Staining of human kidney (fetal, FFPE) using frizzled-10 antibody Cat-No. SP4143P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 6: Staining of human kidney (fetal, FFPE) using frizzled-10 antibody Cat-No. SP4143P; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen

References

Milovanovic T et al: Expression of Wnt genes and frizzled 1 and 2 receptors in normal breast epithelium and infiltrating breast carcinoma.
Int J Oncol, Nov 2004; 25(5): 1337-1342. [PMID: 15492823]
Malbon CC. Frizzleds: new members of the superfamily of G-protein-coupled receptors.
Front Biosci, May 2004, 1; 9: 1048-1058. [PMID: 14977528]
Huang HC et al: The frizzled family: receptors for multiple signal transduction pathways.
Genome Biol, 2004; 5(7): 234. [PMID: 15239825]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD349 / FZD9 / Frizzled-9 (N-term, Extracel. Dom.) antibody

Immunohistochemistry: AP06970PU-N FZD9 antibody staining of Formalin-Fixed Paraffin-Embedded Human Testis at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Bear, Bov, Can, Fi, Hu, Ms, Op, Platypus, Prim, Rb, Rt, Tr E, P
50 µg / €470.00
  Acris Antibodies GmbH

CD349 / FZD9 / Frizzled-9 (3rd Extracell. Dom.) antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Can, Hst, Hu, Ms, Op, Prim, Rb, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

CD349 / FZD9 / Frizzled-9 (N-term, Extracel. Dom.) antibody

Immunohistochemical staining of Brain (Purkinje neurons) using anti- FZD9 antibody SP4154P Rabbit Aff - Purified Bov, Can, Chk, Hst, Hu, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Ze ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

FZD1 / Frizzled-1 (C-term) antibody

Western blot analysis of anti-FZD1 Antibody (C-term) (Cat.#AP12393PU-N) in Jurkat cell line lysates (35ug/lane). FZD1 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FZD2 / Frizzled-2 antibody

Western blot analysis of Frizzled-2 antibody (Cat.-No:
 AP01425PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FZD3 / Frizzled-3 antibody

Western blot (WB) analysis of Frizzled-3 antibody (Cat.-No.: AP01205PU-N) in extracts from A549 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FZD5 / Frizzled-5 antibody

Western blot (WB) analysis of Frizzled-5 antibody (Cat.-No.: AP01316PU-N) in extracts from LOVO cells. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FZD5 / Frizzled-5 (N-term, Extracell. Dom.) antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Can, Elephant, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

FZD6 (N-term, Extracel. Dom.) antibody

Immunohistochemical staining of Pancreas using anti- FZD6 antibody SP4147P Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hu, Kangaroo Rt, L, Mky, Ms, Op, Por, Prim, Rb, Tr C, ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

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