Antibodies to Gastrin-releasing Peptide (Bombesin) and its Receptors (BRS-3, GRP-R and Neuromedin B-Receptor) - FocusOn 073
Introduction: Bombesin (BN) is a tetradecapeptide (Pyro-ENR LGN QWA VGH LM-amide) that was originally isolated from frog skin.
Several BN-related peptides have been isolated from amphibians and classified into three subfamilies: Bombesin, ranatensin, and phyllolitorin based upon the last three C-terminal residues. Two BN-like peptides have been identified in mammals: NMB (neuromedin B) in the ranatensin family and GRP (gastrin-releasing peptide) in the BN-family. BN-family of G-protein coupled receptors include at least four receptor subtypes: The GRP-preferring receptors (GRP-R or bb2), the NMB-preferring receptors (NMB-R or bb1) and Bombesin receptor subtype 3 (BRS-3 or bb4). These receptors share approx. 50% amino acid homology and bind bombesin. However, BRS-3 has much lower affinity for BN than GRP-R and NMB-R. Mammalian BN-like peptides are widely distributed in the brain and gastrointestinal tract, where they modulate smooth-muscle contraction, exocrine and endocrine activities, metabolism and behavior. Most recently, BRS-3 deficient mice have been shown to develop mild obesity, associated with hypertension, and impairment of glucose metabolism, reduced metabolic rate, increased feeding efficiency and subsequent hyperphagia. Human BRS-3 gene (chromosome x) encodes a 399 aa protein with seven transmembrane domains. The NH2 and COOH-termini are predicted to be extracellular and cytoplasmic, respectively.
Antibody Tools for the Detection of Bombesin and Bombesin Receptors
Acris Antibodies offers a range of antibodies for detection of Bombesin and its receptors (GRP-R, BRS-3 and Neuromedin B Receptor). Products can be used for immunological applications like immunohistochemistry (see pictures below).
Fig.1: Staining of rat dorsal horn using anti Bombesin antibody Cat.-No. 20073.
Fig.2: Staining of Pancreas (Islet of Langerhans) using anti GRP-R antibody Cat.-No. SP4337P
Fig.3: Cat.-No. SP4598P Neuromedin B receptor antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Pituitary.
Fig.4: Cat.-No. SP4074P BRS3 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Placenta, Villi.
Fig.5: Cat.-No. SP4075P BRS3 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Placenta, Villi.
Available Antibody Panel to Gastrin-releasing Peptide (Bombesin) and its Receptors
Anti- Bombesin (Gastrin-releasing Peptide, GRP), Neuromedin B receptor (NMBR), Gastrin-Releasing Peptide Receptor (GRPR), Bombesin Receptor Subtype 3 (BRS3) antibody
|Rabbit||Serum||Amph, Mam||C, ICC/IF||
0.1 ml / €390.00
|Rabbit||Aff - Purified||Bear, Bov, Bt, Can, Eq, GP, Hu, Mky, Ms, Prim, Rb, Rt, Sh||E, P||
50 µg / €470.00
|Acris Antibodies GmbH|