Antibodies to Kallikrein Family - FocusOn 006

Introduction: KLK11, KLK12, KLK13, KLK 14 and KLK15, KLK2, KLK3, KLK5 and KLKL1- KLKL4

AP20004PU-N Kallikrein-7/KLK7 antibody staining of Human Paraffin-Embedded Cal27 xenograft at 1/100 dilution.
Fig.1: Cat.-No. AP20004PU-N Kallikrein-7/KLK7 antibody staining of Human Paraffin-Embedded Cal27 xenograft at 1/100 dilution.
SM3072P Kallikrein-3/PSA antibody staining of Paraffin-Embedded Human Prostate Section.
Fig.2: Cat.-No.SM3072P Kallikrein-3/PSA antibody staining of Paraffin-Embedded Human Prostate Section.

Kallikrein (KLK) enzymes are a group of serine proteases found in many different tissues and body fluids. There are two main types of kallikreins, plasma and tissue kallikreins. Plasma kallikreins are only expressed by liver cells. They are involved in various processes such as blood clotting and regulation of vascular tone. Tissue kallikreins have been characterised in rodents and humans to be a highly conserved multi-gene family. The human kallikrein gene family has around 15 known genes that show remarkable similarity at the gene and protein level. In addition 12 related genes have been identified on an adjacent locus to the human kallikrein gene family. Within this large family, some members are of particular interest for diagnostic applications. KLK3, better known as Prostate specific antigen (PSA), is well widely used as a relatively sensitive and specific marker for prostata carcinomas. Besides KLK2 and KLK10, KLK6 and KLK11 are in discussion of potential markers for prostate and ovarian carcinomas. Kallikrein 6 is broadly expressed in glandular epithelia. In the brain, it has been located in the epithelial cells of the choroid plexus. Soluble kallikrein 6 has also recently been reported as a potential marker for Alzheimer’s disease, with bloodlevels being increased significantly over controls. Kallikrein 11 is expressed in brain, skin, salivary gland, stomach, uterus, lung,thymus, colon, breast, thyroid, ovary and prostate. Increased levels are reported in prostate and ovarian carcinomas.

AP20016PU-N Kallikrein-14/KLK14 antibody staining of Palmar Skin. The KLK14 antibody stains intraepidermal sweat ducts.
Fig.3: Cat.-No. AP20016PU-N Kallikrein-14/KLK14 antibody staining of Palmar Skin. The KLK14 antibody stains intraepidermal sweat ducts.
Kallikrein-5/KLK5 antibody staining of Human Prostate lysate at 0.1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Fig.4: Kallikrein-5/KLK5 antibody staining of Human Prostate lysate at 0.1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Antibody Tools for Detection of Kallikreins

Acris Antibodies offers a comprehensive antibody panel to the Kallikrein family. These antibodies are useful for the detection of Kallikreins by Western blotting. Some of the antibodies to Kallikrein 3 (KLK3, PSA) can also be used in ELISA or immunohistochemical staining.

Available antibody panel to Kallikrein (KLK) enzymes

Kallikrein 11 (KLK11 / Hippostatin), Kallikrein 12 (KLK12), Kallikrein 13 (KLK13), Kallikrein 14 (KLK14), Kallikrein 15 (KLK15), Kallikrein 2 (KLK2), Kallikrein 3 (KLK3, PSA) Kallikrein 4 (KLK4 / Kallikrein L1 / KLK-L1), Kallikrein 5 (KLK5), Kallikrein 9 (KLK9 / Kallikrein L3 / KLK-L3, Kallikrein L2 (KLK-L2), Kallikrein L4 antibody (KLK-L4)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Kallikrein-3 / PSA / KLK3 antibody

Western Blot: Detection of  PSA antigen (100ng/well) using 1ug/mL mouse anti-human PSA mAb clone CHYH1. Mouse IgG1 CHYH1 Purified Hu C, E, E(capture), P, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Kallikrein-3 / PSA / KLK3 Mouse IgG1 58.10D6 Purified Hu E, E(capture)
0.2 mg / €360.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 (C-term) antibody

Western blot analysis of anti-KLK3 Pab in mouse brain tissue lysate.KLK3(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Kallikrein-3 / PSA / KLK3 Mouse IgG1 214 Purified Hu E, E(detection)
1 mg / €240.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Kallikrein-3 / PSA / KLK3 Mouse IgG2a 251 Purified Hu E, E(capture)
1 mg / €490.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Western Blot: Detection of PSA antigen (100ng/well) using 1ug/mL mouse anti-human PSA mAb clone CHYH2. Mouse IgG1 CHYH2 Purified Hu C, E, P, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Kallikrein-3 / PSA / KLK3 Mouse IgG1 CHYH2 HRP Hu C, E, P
0.2 ml / €460.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Figure.1: FACS analysis of BOSC23 cells using LT-3D2 antibody(Cat.#DM1225). BOSC23 cells were transiently transfected with anexpression vector encoding either PSA (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of LT-3D2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSA trans-fected cells. Mouse IgG1 LT-3D2 Purified Hu E, F
0.1 mg / €330.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Formalin fixed paraffin embedded human prostate stained with PSA antibody. DP033 Rabbit Purified Hu C, P
0.5 ml / €230.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 antibody

Figure 1. SM3072P Kallikrein-3/PSA antibody staining of Paraffin-Embedded Human Prostate Section. Mouse IgG1 A67-B/E3 Purified Hu E, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

KLK2 / Kallikrein-2 (C-term) antibody

Western blot analysis of Kallikrein 2 (KLK2) Antibody (C-term) in K562 cell line lysates (35ug/lane). KLK2 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KLK5 / Kallikrein-5 antibody

Western blot analysis of anti-KLK5 Pab in mouse brain tissue lysate.KLK5(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KLK5 / Kallikrein-5 antibody

Figure 1. Western Blot of 10 µl conditional medium reduced by beta-Mercaptoethanol using KLK5 antibody (1/1000). The antibody is conjugated to Alkaline Phosphatase. Rabbit Serum Hu WB
0.2 ml / €390.00
  Acris Antibodies GmbH

KLK5 / Kallikrein-5 (pro-) antibody

Figure 1. Western Blot of 10 µl conditional medium  using pro-KLK5 antibody (1/1000).
Lane 1: Debatured Protein.
Lane 2: Native Protein.
The antibody is conjugated to Alkaline Phosphatase. Rabbit Serum Hu WB
0.2 ml / €390.00
  Acris Antibodies GmbH

KLK9 / Kallikrein-9 (N-term) antibody

Western blot analysis of anti-KLK9 Pab in mouse brain tissue lysate.KLK9(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

KLK14 / Kallikrein-14 antibody

Figure 1. AP20016PU-N Kallikrein-14/KLK14 antibody staining of Palmar Skin. The KLK14 antibody stains intraepidermal sweat ducts. Rabbit Aff - Purified Hu C, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

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