Antibodies to MAP Kinases - FocusOn 096

Many different stimuli such as growth factors, cytokines, viral infection, ligands for G protein-coupled receptors and transforming agents activate the mitogen-activated protein kinase (MAPK) pathway.

MAPK is also called extracellular signal regulated kinase (ERK). Activation of the MAPK pathway is mediated via monomeric GTPases of the RAS-family. RAS is able to transmit cellular signals from G protein-coupled receptors as well as from receptor (associated) thyrosine kinases, which in turn are mediated via adaptor molecules like Grb2/SOS.

RAS activates the so-called MAPKKK (MAPK kinase kinase) with c-Raf (RAF1) being one of the best described members of this group. Once activated, c-Raf phosphorylates two serine residues in the downstream MAPKK, also called MAP/ERK kinase (MEK). MEK1 and 2 are dual specificity protein kinases and phophorylate the following MAP kinases, the best described members being ERK1 and ERK2, at tyrosine and threonine residues. All MAP kinases known are characterized by their requirement for this dual phosphorylation for full activation at conserved T-X-Y motif.

Upon activation, the MAPKs/ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate transcription factors such as c-Myc and ELK-1.

Today, many different MAPKs/ERKs and MEKs are known and each are activated by different upstream stimuli like growth factors, UV-mediated stress, cytokines or even cell-cell interaction. Other well-described MAPKs are c-Jun N-terminal kinases (JNKs) and members of the p38-MAPK family.

Fig. 1: Human prostate (formalin-fixed, paraffin-embedded, FFPE) stained with Acris MAPK3 / ERK1 antibody Cat.-No. AP03060PU-N at 4 µg/ml
Fig. 2: Human prostate (FFPE) stained with Acris MAPK1/ERK2 antibody Cat.-No.AP08376PU-N at 2.5 µg/ml
Fig. 3: Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) SKOV3 tumor cells applied to SDS-PAGE (ca 20,000 cells per lane) and transferred to a PVDF membrane. The WB was probed with Acris ERK1/2 antibody clone 12D4 Cat.-No. AM00084PU-N
Fig. 4: Detection of endogenous MAPK7/ERK5 whole cell lysates of serum starved tumor cells (20,000 cells per lane) applied to SDS-PAGE and transferred to a PVDF membrane. WB probed with Acris ERK5 antibody clone 12F2 Cat.-No. AM00058PU-N (0.5 µg/ ml): Lane 1: A431; Lane 2: A549; Lane 3: SKOV3; Lane 4: OVCAR5; Lane 5: HaCaT; Lane 6: PC3; Lane 7: HeLa; Lane 8: HepG2
Fig. 5: Human hepatocarcinoma tissue (FFPE) reacted with Acris MEK1 antibody Cat.-No. AP14764PU-N, which was peroxidase-conjugated to a secondary antibody followed by DAB staining
Fig. 6: Human liver cancer tissue (FFPE) reacted with Acris MEK2 antibody Cat.-No. AP14764PU-N, peroxidase-conjugated secondary antibody / AEC staining

Selected Antibody Panel to ERK1–5 and MEK1/2

Acris Antibodies offers a range of thoroughly evaluated monoclonal and polyclonal antibodies to different members of the MAP kinase cascade, including products for immunohistochemical applications on frozen or paraffin embedded sections, immunofluorescence and/or Western blotting. This collection is extended by Abnova antibodies distributed by Acris Antibodies within Europe and the USA. Further products, e.g. antibodies to c-Raf, JNK and p38 MAPK can be found via the above input field ‘Search’.

Key References

 
Ji RR et al.: p38 MAPK, microglial signaling, and neuropathic pain.
Mol Pain, Nov 2007; 1: 3:33
 
Fischer AM et al.: The role of Erk1 and Erk2 in multiple stages of T cell development.
Immunity, Oct 2005; 23(4): 431-443.
 
Seternes OM et al.: Activation of MK5/PRAK by the atypical MAP kinase ERK3 defines a novel signal transduction pathway.
EMBO J, Dec 2004; 8; 23(24): 4780-4791. Erratum in: EMBO J, Feb 2005 23; 24(4): 873.
 
Sohn SJ et al.: Transcriptional regulation of tissue-specific genes by the ERK5 mitogen-activated protein kinase.
Mol Cell Biol, Oct 2005; 25(19): 8553-8566.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERK1 / ERK2 antibody

ERK1 / ERK2 Mouse IgG2a SB46b Biotin Hu IP, WB
0.5 mg / €300.00
  Acris Antibodies GmbH

ERK1 / ERK2 antibody

ERK1 / ERK2 Mouse IgG2a SB46b HRP Hu IP, WB
1 ml / €300.00
  Acris Antibodies GmbH

ERK1 / ERK2 antibody

Western Blot or Immunoprecipitation: Human PBMC and Spleen Lysate was stained with Mouse Anti-Erk1/2 (AM08205PU-N). Mouse IgG2a SB46b Purified Hu IP, WB
50 µg / €300.00
  Acris Antibodies GmbH

ERK1 / ERK2 pThr202 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase antibody (pThr202) Cat.-No AP02498PU (left) or the same antibody preincubated with Blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

ERK1 / ERK2 pThr202 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase antibody (pThr202) Cat.-No AP02498PU (left) or the same antibody preincubated with Blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

ERK1 / ERK2 pThr187/pTyr204 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p44/42 MAP Kinase antibody (AP02499PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

ERK1 / ERK2 pThr187/pTyr204 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p44/42 MAP Kinase antibody (AP02499PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

ERK1 / ERK2 antibody

Immunohistochemical analysis of paraffin-embedded human Colorectal tissue using p44/42 MAP Kinase Antibody Cat.-No AP02741PU Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Acris Antibodies GmbH

ERK1 / ERK2 antibody

Immunohistochemical analysis of paraffin-embedded human Colorectal tissue using p44/42 MAP Kinase Antibody Cat.-No AP02741PU Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €230.00
  Acris Antibodies GmbH

ERK1 / ERK2 antibody

Western blot analysis of extracts from NIH-3T3 cells, using p44/42 MAP Kinase antibody (Line 1 and 2) and p44/42 MAP Kinase (pTyr204) antibody (Line 3). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

ERK1 / ERK2 antibody

Western blot analysis of extracts from NIH-3T3 cells, using p44/42 MAP Kinase antibody (Line 1 and 2) and p44/42 MAP Kinase (pTyr204) antibody (Line 3). Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

ERK2 (N-term) (incl. pos. control) antibody

Detection of endogenous MAPK2: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with  MAPK2 antibody (0.5 μg/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: A431 
Lane 2:SW480 
Lane 3: SW620 
Lane 4: HT29 
Lane 5: MCF7 
Lane 6: MDA231 
Lane 7: T47D Mouse IgG1 6H3 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ERK2 antibody

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ERK2 antibody

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

ERK2 antibody

Detection limit for recombinant GST tagged MAPK1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 1D1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERK2 antibody

Human breast carcinoma stained with anti-ERK-2 antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €440.00
  Acris Antibodies GmbH

ERK2 antibody

Human breast carcinoma stained with anti-ERK-2 antibody Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

ERK2 (C-term) (incl. pos. control) antibody

ERK2 Mouse IgG1 6G11 Biotin Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

ERK2 (C-term) (incl. pos. control) antibody

Detection of endogenous MAPK2: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MAPK2-6G11 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: A431 
Lane 2: A549 
Lane 3: SKOV3 
Lane 4: OVCAR5 
Lane 5: HaCaT  
lane 6: PC3 
Lane 7: HeLa
Lane 8: HepG2 Mouse IgG1 6G11 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ERK2 (N-term) (incl. pos. control) antibody

ERK2 Mouse IgG1 6H3 Biotin Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

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