Antibodies to p53 and p53 Interacting Proteins - FocusOn 129

Selected Antibody Tools for the Detection of p53 and p53 Interacting Proteins

Acris Antibodies offers a wide range of antibodies to p53 and p53 interacting proteins for various applications like Immunoprecipitation, Immunohistochemistry, ELISA or Western blotting (including phospho-specific antibodies). P53 control peptides are also available (see Product list).

p53

Cellular tumor antigen p53 is also called tumor protein p53 (TP53) or transformation-related protein 53 (TRP53). p53 acts as a tumor suppressor in a wide variety of tissue types, and is involved in regulating cell growth, replication and apoptosis. p53 is mutated in approximately half of all human malignancies including breast, colon and lung cancers. Li-Fraumeni syndrome (LFS) is a genetic autosomal dominant cancer syndrome – a rare disorder that greatly increases the risk of developing several types of cancer, particularly in children and young adults; the majority of LFS (~ 70 %) is caused by mutations in p53. In normal, undamaged cells, p53 is rapidly degraded. Treating cells with a variety of DNA damage-inducing agents induces a transient accumulation of p53 protein and activates it as a transcription factor. Human p53 protein has been shown to be phosphorylated at several N-terminus and C-terminus sites that in vitro affects site-specific DNA binding and interaction with other cellular and viral proteins. Serines 6, 9, 15, 20, 33, and 37 phosphorylation occurs after cells are exposed either to ionizing radiation or to UV light. Ser6 and Ser15 were demonstrated to be one of the strongest and earliest phosphorylated sites in response to DNA damage-induced posttranslational modifications.

Fig. 1: Staining of Wild-type p53 expressed in formalin-fixed, paraffin-embedded (FFPE) human trophoblast sections. 1a: anti-p53 antibody (total), 1b: anti-p53 antibody clone FP3.2 (phospho Ser392) Cat.-No. AM03100PU-N
Fig. 1: Staining of Wild-type p53 expressed in formalin-fixed, paraffin-embedded (FFPE) human trophoblast sections. 1a: anti-p53 antibody (total), 1b: anti-p53 antibody clone FP3.2 (phospho Ser392) Cat.-No. AM03100PU-N

Fig. 2: Staining of human colon carcinoma tissue (FFPE) with p53 antibody Cat.-No. DB 026-0.5 shows positive nuclear immunostaining of tumor cells
Fig. 2: Staining of human colon carcinoma tissue (FFPE) with p53 antibody Cat.-No. DB 026-0.5 shows positive nuclear immunostaining of tumor cells
Fig. 3: Staining of human colon carcinoma tissue (FFPE) with p53 antibody Cat.-No AM11182PU-N
Fig. 3: Staining of human colon carcinoma tissue (FFPE) with p53 antibody Cat.-No AM11182PU-N
Fig. 4: Confocal microscopy of human HeLa cells using p53 antibody clone BP53-12 Cat.-No. BM4079F (FITC)
Fig. 4: Confocal microscopy of human HeLa cells using p53 antibody clone BP53-12 Cat.-No. BM4079F (FITC)
Fig. 5: Staining of human colon carcinoma (FFPE) with p53 antibody Cat.-No. DP106
Fig. 5: Staining of human colon carcinoma (FFPE) with p53 antibody Cat.-No. DP106

p53AIP

The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. p53-regulated apoptosis-inducing protein 1 (p53AIP) was identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser46 (Oda, 2000). Ectopic expression of p53AIP leads to apoptotic cell death.

p53DINP1

A p53 inducible gene was identified and designated p53DINP1 (p53-dependent damage-inducible nuclear protein 1) and SIP (for stress induced protein) in human and mouse. A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks (Okamura, 2001). p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress (Tomasini, 2001).

Fig. 6. Western blot analysis of p53DINP1 expression in human lung tissue lysate with p53DINP1 antibody Cat.-No. AP30639PU-N at 0.5 µg/ml (A) and 1 µg/ml (B)
Fig. 6. Western blot analysis of p53DINP1 expression in human lung tissue lysate with p53DINP1 antibody Cat.-No. AP30639PU-N at 0.5 µg/ml (A) and 1 µg/ml (B)
Fig. 7: Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using p53BP1 antibody Cat.-No. AP22392PU-N at 1/200 dilution
Fig. 7: Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using p53BP1 antibody Cat.-No. AP22392PU-N at 1/200 dilution

p53R2

p53-inducible ribonucleotide reductase (RR) small subunit 2-like protein was identified as a RR gene which is directly regulated by p53 protein in the G1 and G2 phases of the cell cycle supplying nucleotides to repair damaged DNA.

Key references

Boularan C et al: Beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53.
Proc Natl Acad Sci U S A, Nov 2007; 104(46): 18061-6. PMID17984062
Hideki H et al: Infrequent alteration in the p53R2 gene in human transitional cell carcinoma of the urinary tract.
Pathobiology 2004; 71: 103-106. PMID14707445
Oda Ket al: p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53.
Cell, Sep 2000; 102(6): 849-62. PMID11030628
Okamura S et al: p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis.
Mol Cell, Jul 2001; 8(1): 85-94. PMID11511362
Poeta ML et al: TP53 mutations and survival in squamous-cell carcinoma of the head and neck.
N Engl J Med, Dec 2007; 357(25): 2552-61. PMID18094376
Stagno D’Alcontres M et al: Lack of TRF2 in ALT cells causes PML-dependent p53 activation and loss of telomeric DNA.
J Cell Biol, Dec 2007; 179(5): 855-67. PMID18056407
Tomasini R et al: Molecular and functional characterization of the stress-induced protein (SIP) gene and its two transcripts generated by alternative splicing. SIP induced by stress and promotes cell death.
J Biol Chem, Nov 2001; 276(47): 44185-92. PMID11557757

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RRM2B / P53R2 (2-17) antibody

Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Rabbit Aff - Purified Eq, Hu, Mky, Ms, Prim, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RRM2B / P53R2 Control Peptide

RRM2B / P53R2
50 µg / €160.00
  Acris Antibodies GmbH

TP53AIP1 (N-term) antibody

AP07718PU-N P53AIP1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TP53AIP1 Control Peptide

TP53AIP1
50 µg / €160.00
  Acris Antibodies GmbH

TP53BP2 antibody

Immunoperoxidase of monoclonal antibody to TP53BP2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml] Mouse IgG2a 3F8 Purified Hu E, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

TP53INP1 antibody

Western blot analysis of p53DINP1 expression in Human lung tissue lysate. Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

TP53INP1 (N-term) antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

TP53INP1 Control Peptide

TP53INP1
50 µg / €160.00
  Acris Antibodies GmbH

TP53 / p53 pSer392 antibody

Figure 1. Immunohistochemistry staining of Wild-type p53 expressed in Human Trophoblast (Paraffin-Embedded Sections): 1A: anti-p53 antibody (Total), 1B: anti-p53 antibody (Phospho Ser392). 
Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive. Mouse IgG1 FP3.2 Aff - Purified Hu P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

TP53 / p53 pSer6 antibody

Western blot analysis of extracts from HT29 and Hela cells untreated or treated with UV using p53(Phospho-Ser6) Antibody Cat.-No AP08001PU Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

TP53 / p53 (16-25) antibody

Confocal Microscopy of Human HeLa cells using BM4079F p53 antibody (BP53-12, FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC antibody. Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic. Mouse IgG2a BP53-12 Purified Hu, Prim C, E, ICC/IF, IP, P, WB
0.1 mg / €220.00
  Acris Antibodies GmbH

TP53 / p53 antibody

Human colon carcinoma stained with Anti-p53 antibody Mouse IgG2a SPM514 Purified Hu P
1 ml / €500.00
  Acris Antibodies GmbH

TP53 / p53 antibody

Human colon carcinoma stained with Anti-p53 antibody Mouse IgG2a SPM514 Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

TP53 / p53 antibody

AM11182PU-N p53 antibody staining of Formalin-Fixed, Paraffin-Embedded Human colon carcinoma tissue. Rabbit IgG SP5 Supernatant Bov, Can, Hst, Hu, Ms, Por, Rb, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

TP53 / p53 antibody

AM11182PU-N p53 antibody staining of Formalin-Fixed, Paraffin-Embedded Human colon carcinoma tissue. Rabbit IgG SP5 Supernatant Bov, Can, Hst, Hu, Ms, Por, Rb, Rt P
0.1 ml / €290.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type) antibody

AP15706PU p53 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon carcinoma tissue. Rabbit Purified Hu P
1 ml / €330.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type) antibody

AP15706PU p53 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon carcinoma tissue. Rabbit Purified Hu P
0.1 ml / €170.00
  Acris Antibodies GmbH

TP53 / p53 pSer392 antibody

Detection of activated p53 Recombinant C-terminal fragment of p53 was incubated with Casein Kinase II in the  absence (1) or presence (2) of ATP. Proteins were separarted by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Monoclonal p53 antibody (clone 9F4) (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 9F4 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

TP53 / p53 (Wild type + Mutant) (20-31) antibody

TP53 / p53 Mouse IgG2a Bp53-11 Aff - Purified Hu C, E, ICC/IF, IP, P, WB
2x25 µg / €290.00
  Acris Antibodies GmbH

TP53 / p53 pSer15 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser15) antibody (AP02390PU). Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

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